Extra centrosomes and/or chromosomes prolong mitosis in human cells
Extra centrosomes and/or chromosomes prolong mitosis in human cells
Date
2008-04-04
Authors
Yang, Zhenye
Loncarek, Jadranka
Khodjakov, Alexey
Rieder, Conly L.
Loncarek, Jadranka
Khodjakov, Alexey
Rieder, Conly L.
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Keywords
Mitosis
Centrosomes
Chromosomes
Cancer
Spindle assembly checkpoint
Centrosomes
Chromosomes
Cancer
Spindle assembly checkpoint
Abstract
Using laser microsurgery and cell fusion we have explored how additional
centrosomes and/or chromosomes influence the duration of mitosis in human cells. We
find that doubling the chromosome number adds ~10 minutes to a 20 minute division
while doubling the number of centrosomes adds ~30 minutes more, and extra
centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the
spindle assembly checkpoint. Thus mitosis can be prolonged by non genetic means and
extra chromosomes and centrosomes likely contribute to the elevated mitotic index seen
in many tumors.
Description
Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature Cell Biology 10 (2008): 748-751, doi:10.1038/ncb1738.