Transgenerational marking of embryonic otoliths in marine fishes using barium stable isotopes Thorrold, Simon R. Jones, Geoffrey P. Planes, Serge Hare, Jonathan A. 2007-01-11T21:45:01Z 2007-01-11T21:45:01Z 2006-04-13
dc.description Author Posting. © National Research Council Canada, 2006. This article is posted here by permission of National Research Council Canada for personal use, not for redistribution. The definitive version was published in Canadian Journal of Fisheries and Aquatic Sciences 63 (2006): 1193-1197, doi:10.1139/F06-048. en
dc.description.abstract We describe a new technique for transgenerational marking of embryonic otoliths that promises significant advancements in the study of larval dispersal and population connectivity in marine fishes. The approach is based on maternal transmission of 137Ba from spawning females to egg material that is ultimately incorporated into the otoliths of embryos produced by an individual after exposure to the isotope. We injected females of a benthic-spawning clownfish (Amphiprion melanopus) and a pelagic-spawning serranid (Centropristis striata) with enriched 137BaCl2 and then reared the resulting progeny through to settlement. Barium isotope ratios in the cores of larval otoliths were quantified using laser ablation inductively coupled plasma mass spectrometry. Larval otoliths from both species contained unequivocal Ba isotope signatures over a wide range of doses (0.8–23 μg 137Ba·g female–1). Female A. melanopus continued to produce marked larvae over multiple clutches and for at least 90 days after a single injection. The ability to administer different combinations of stable Ba isotopes provides a new means of mass-marking larvae of benthic- and pelagic-spawning fishes from multiple populations over extended spawning periods. en
dc.description.sponsorship Funding for this study was provided by the National Science Foundation through grants OCE-0215905 and OCE- 0424688, Discovery Grant DP 0208120 from the Australian Research Council, and the Oak Foundation. en
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dc.identifier.citation Canadian Journal of Fisheries and Aquatic Sciences 63 (2006): 1193–1197 en
dc.identifier.doi 10.1139/F06-048
dc.language.iso en en
dc.publisher NRC Research Press en
dc.title Transgenerational marking of embryonic otoliths in marine fishes using barium stable isotopes en
dc.type Article en
dspace.entity.type Publication
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