Optimization of a GCaMP calcium indicator for neural activity imaging
Optimization of a GCaMP calcium indicator for neural activity imaging
dc.contributor.author | Akerboom, Jasper | |
dc.contributor.author | Chen, Tsai-Wen | |
dc.contributor.author | Wardill, Trevor J. | |
dc.contributor.author | Tian, Lin | |
dc.contributor.author | Marvin, Jonathan S. | |
dc.contributor.author | Mutlu, Sevinc | |
dc.contributor.author | Calderon, Nicole Carreras | |
dc.contributor.author | Esposti, Federico | |
dc.contributor.author | Borghuis, Bart G. | |
dc.contributor.author | Sun, Xiaonan Richard | |
dc.contributor.author | Gordus, Andrew | |
dc.contributor.author | Orger, Michael B. | |
dc.contributor.author | Portugues, Ruben | |
dc.contributor.author | Engert, Florian | |
dc.contributor.author | Macklin, John J. | |
dc.contributor.author | Filosa, Alessandro | |
dc.contributor.author | Aggarwal, Aman | |
dc.contributor.author | Kerr, Rex A. | |
dc.contributor.author | Takagi, Ryousuke | |
dc.contributor.author | Kracun, Sebastian | |
dc.contributor.author | Shigetomi, Eiji | |
dc.contributor.author | Khakh, Baljit S. | |
dc.contributor.author | Baier, Herwig | |
dc.contributor.author | Lagnado, Leon | |
dc.contributor.author | Wang, Samuel S.-H. | |
dc.contributor.author | Bargmann, Cornelia I. | |
dc.contributor.author | Kimmel, Bruce E. | |
dc.contributor.author | Jayaraman, Vivek | |
dc.contributor.author | Svoboda, Karel | |
dc.contributor.author | Kim, Douglas S. | |
dc.contributor.author | Schreiter, Eric R. | |
dc.contributor.author | Looger, Loren L. | |
dc.date.accessioned | 2012-10-12T19:50:34Z | |
dc.date.available | 2012-10-12T19:50:34Z | |
dc.date.issued | 2012-10-03 | |
dc.description | © The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Neuroscience 32 (2012): 13819-13840, doi:10.1523/JNEUROSCI.2601-12.2012. | en_US |
dc.description.abstract | Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of GECIs. The state-of-the art single-wavelength GECI, GCaMP3, has been deployed in a number of model organisms and can reliably detect three or more action potentials in short bursts in several systems in vivo. Through protein structure determination, targeted mutagenesis, high-throughput screening, and a battery of in vitro assays, we have increased the dynamic range of GCaMP3 by severalfold, creating a family of “GCaMP5” sensors. We tested GCaMP5s in several systems: cultured neurons and astrocytes, mouse retina, and in vivo in Caenorhabditis chemosensory neurons, Drosophila larval neuromuscular junction and adult antennal lobe, zebrafish retina and tectum, and mouse visual cortex. Signal-to-noise ratio was improved by at least 2- to 3-fold. In the visual cortex, two GCaMP5 variants detected twice as many visual stimulus-responsive cells as GCaMP3. By combining in vivo imaging with electrophysiology we show that GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3. GCaMP5 allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general. | en_US |
dc.description.sponsorship | A.F. has been supported by a European Molecular Biology Organization long-term fellowship. Work in H.B.’s laboratory was funded by the National Institutes of Health (NIH) Nanomedicine Development Center “Optical Control of Biological Function,” and work in S.S.-H.W.’s laboratory was funded by NIH R01 NS045193. | en_US |
dc.format.mimetype | application/pdf | |
dc.identifier.citation | Journal of Neuroscience 32 (2012): 13819-13840 | en_US |
dc.identifier.doi | 10.1523/JNEUROSCI.2601-12.2012 | |
dc.identifier.uri | https://hdl.handle.net/1912/5448 | |
dc.language.iso | en_US | en_US |
dc.publisher | Society for Neuroscience | en_US |
dc.relation.uri | https://doi.org/10.1523/JNEUROSCI.2601-12.2012 | |
dc.rights | Attribution-NonCommercial-ShareAlike 3.0 Unported | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | * |
dc.title | Optimization of a GCaMP calcium indicator for neural activity imaging | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
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