Diversity and distribution of subseafloor Thermococcales populations in diffuse hydrothermal vents at an active deep-sea volcano in the northeast Pacific Ocean

dc.contributor.author Huber, Julie A.
dc.contributor.author Butterfield, David A.
dc.contributor.author Baross, John A.
dc.date.accessioned 2007-02-05T16:43:00Z
dc.date.available 2007-02-05T16:43:00Z
dc.date.issued 2006-12-20
dc.description Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 111 (2006): G04016, doi:10.1029/2005JG000097. en
dc.description.abstract The presence, diversity, and distribution of a key group of subseafloor archaea, the Thermococcales, was examined in multiple diffuse flow hydrothermal vents at Axial Seamount, an active deep-sea volcano located in the northeast Pacific Ocean. A polymerase chain reaction (PCR) approach was used to determine if this group of subseafloor indicator organisms showed any phylogenetic distribution that may indicate distinct subseafloor communities at vents with different physical and chemical characteristics. Targeted primers for the Thermococcales 16S rRNA (small subunit ribosomal RNA) gene and intergenic transcribed spacer (ITS) region were designed and applied to organisms filtered in-situ directly from a variety of diffuse flow vents. Thermococcales were amplified from 9 of 11 samples examined, and it was determined that the ITS region is a better phylogenetic marker than the 16S rRNA in defining consistent groups of closely related sequences. Results show a relationship between environmental clone distribution and source vent chemistry. The most highly diluted vents with elevated iron and alkalinity contained a distinct group of Thermococcales as defined by the ITS region, suggesting separate subseafloor Thermococcales populations at diffuse vents within the Axial caldera. en
dc.description.sponsorship This work was supported by Washington Sea Grant (NA76RG0119), National Science Foundation (OCE 9816491), NSF IGERT (DGE- 9870713), NASA Astrobiology Institute through the Carnegie Geophysical Institute, the NOAA/PMEL Vents Program, NOAA West Coast and Polar Undersea Research Center, and by the Joint Institute for the Study of the Atmosphere and Ocean under NOAA Cooperative Agreement No. NA117RJ1232. en
dc.format.mimetype application/pdf
dc.identifier.citation Journal of Geophysical Research 111 (2006): G04016
dc.identifier.doi 10.1029/2005JG000097
dc.identifier.uri https://hdl.handle.net/1912/1435
dc.language.iso en_US en
dc.publisher American Geophysical Union
dc.relation.uri https://doi.org/10.1029/2005JG000097
dc.subject Subseafloor en
dc.subject Hydrothermal vent en
dc.subject Intergenic Transcribed Spacer (ITS) region en
dc.subject Geochemistry en
dc.subject Diffuse flow vent en
dc.title Diversity and distribution of subseafloor Thermococcales populations in diffuse hydrothermal vents at an active deep-sea volcano in the northeast Pacific Ocean en
dc.type Article en
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 6cd11aa1-056c-4869-b5de-fc613c898112
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