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dc.contributor.authorGupton, S. L.
dc.contributor.authorCollings, D. A.
dc.contributor.authorAllen, N. S.
dc.date.accessioned2006-05-24T13:49:19Z
dc.date.available2006-05-24T13:49:19Z
dc.date.issued2005-11-07
dc.identifier.urihttp://hdl.handle.net/1912/952
dc.descriptionAuthor Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Plant Physiology and Biochemistry, doi:10.1016/j.plaphy.2006.03.003.en
dc.description.abstractThe endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.en
dc.description.sponsorshipFunding for this project included NASA grant # NAGW-4984 to the North Carolina NSCORT (NASA Specialized Center of Research and Training) (SLG, DAC, NSA), NSF REU Site Grant #0243930 (NSA), a Sigma Xi Grant-in-Aid Award (SLG), and Australian Research Council Discovery Grant no. DP0208806 (DAC).en
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dc.language.isoen_USen
dc.relation.urihttp://dx.doi.org/10.1016/j.plaphy.2006.03.003
dc.subjectActin microfilamentsen
dc.subjectCell divisionen
dc.subjectEndoplasmic reticulumen
dc.subjectGreen fluorescent proteinen
dc.subjectMicrotubulesen
dc.subjectNuclear envelopeen
dc.subjectNuclear invaginationsen
dc.titleEndoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell divisionen
dc.typePreprinten


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