Subtle microbiome manipulation using probiotics reduces antibiotic-associated mortality in fish
Schmidt, Victor T.
Smith, Katherine F.
Amaral-Zettler, Linda A.
MetadataShow full item record
KeywordAntibiotics; Aquaculture; Bacillus; Colonization resistance; Microbial ecology; Microbiome; Phaeobacter; Probiotics; Vibrio
Prophylactic antibiotics in the aquaculture and ornamental fish industry are intended to prevent the negative impacts of disease outbreaks. Research in mice and humans suggests that antibiotics may disturb microbiome communities and decrease microbiome-mediated disease resistance, also known as “colonization resistance.” If antibiotics impact fish as they do mice and humans, prophylactic administrations on aquaculture farms may increase downstream disease susceptibility in target hosts, despite short-term pathogen control benefits. We tested the effects of antibiotics on mortality after a pathogen challenge in the Poecilia sphenops black molly and subsequently tested if probiotic inoculations could reverse any antibiotic-induced losses of disease resistance. We found that antibiotic treatment significantly increased fish mortality. We further found that our two candidate probiotic bacterial species, Phaeobacter inhibens S4Sm and Bacillus pumilus RI06-95Sm, were able to colonize black molly microbiomes and reverse the negative impacts of antibiotics. Despite the positive impact on survival, probiotic treatment did not influence overall microbiome community structure or diversity. Our results suggest that subtle manipulations of microbiome composition can have dramatic impacts on host phenotype. The results of this study have implications for how antibiotic-treated microbiomes can be restored and suggest that small-scale additions may be as effective as wholesale transplants.
© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in mSystems 2 (2017): e00133-17, doi:10.1128/mSystems.00133-17.
Suggested CitationArticle: Schmidt, Victor T., Gomez-Chiarri, Marta, Roy, Chelsea, Smith, Katherine F., Amaral-Zettler, Linda A., "Subtle microbiome manipulation using probiotics reduces antibiotic-associated mortality in fish", mSystems 2 (2017): e00133-17, DOI:10.1128/mSystems.00133-17, https://hdl.handle.net/1912/9485
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Reproducible community dynamics of the gastrointestinal microbiota following antibiotic perturbation Antonopoulos, Dionysios A.; Huse, Susan M.; Morrison, Hilary G.; Schmidt, Thomas M.; Sogin, Mitchell L.; Young, Vincent B. (2009-03-13)Shifts in microbial communities are implicated in the pathogenesis of a number of gastrointestinal diseases, but we have limited understanding of the mechanisms that lead to altered community structures. One difficulty ...
The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing Dethlefsen, Les; Huse, Susan M.; Sogin, Mitchell L.; Relman, David A. (Public Library of Science, 2008-11-18)The intestinal microbiota is essential to human health, with effects on nutrition, metabolism, pathogen resistance, and other processes. Antibiotics may disrupt these interactions and cause acute disease, as well as ...
Aquarium microbiome response to ninety-percent system water change : clues to microbiome management Van Bonn, William; LaPointe, Allen; Gibbons, Sean M.; Frazier, Angel; Hampton-Marcell, Jarrad T.; Gilbert, Jack A. (2015-04)The bacterial community composition and structure of water from an established teleost fish system was examined before, during and after a major water change to explore the impact of such a water-change disturbance on the ...