• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Early life exposure to low levels of AHR agonist PCB126 (3,3’,4,4’,5- pentachlorobiphenyl) reprograms gene expression in adult brain

    Thumbnail
    View/Open
    Author's manuscript (2.606Mb)
    Supporting data (61.44Kb)
    Supporting data (11.84Mb)
    Supporting data (229.6Kb)
    Supporting data (32.72Kb)
    Supporting data (35.46Kb)
    Date
    2017-09
    Author
    Aluru, Neelakanteswar  Concept link
    Karchner, Sibel I.  Concept link
    Glazer, Lilah  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/9298
    As published
    https://doi.org/10.1093/toxsci/kfx192
    Keyword
     Zebrafish; DOHaD; RNAseq; Latent effects; Brain; Males 
    Abstract
    Early life exposure to environmental chemicals can have long-term consequences that are not always apparent until later in life. We recently demonstrated that developmental exposure of zebrafish to low, non-embryotoxic levels of 3,3’,4,4’,5-pentachlorobiphenyl (PCB126) did not affect larval behavior, but caused changes in adult behavior. The objective of this study was to investigate the underlying molecular basis for adult behavioral phenotypes resulting from early life exposure to PCB126. We exposed zebrafish embryos to PCB126 during early development and measured transcriptional profiles in whole embryos, larvae and adult male brains using RNA-sequencing. Early life exposure to 0.3 nM PCB126 induced cyp1a transcript levels in 2-dpf embryos, but not in 5-dpf larvae, suggesting transient activation of aryl hydrocarbon receptor with this treatment. No significant induction of cyp1a was observed in the brains of adults exposed as embryos to PCB126. However, a total of 2209 and 1628 genes were differentially expressed in 0.3 nM and 1.2 nM PCB126-exposed groups, respectively. KEGG pathway analyses of upregulated genes in the brain suggest enrichment of calcium signaling, MAPK and notch signaling, and lysine degradation pathways. Calcium is an important signaling molecule in the brain and altered calcium homeostasis could affect neurobehavior. The downregulated genes in the brain were enriched with oxidative phosphorylation and various metabolic pathways, suggesting that the metabolic capacity of the brain is impaired. Overall, our results suggest that PCB exposure during sensitive periods of early development alters normal development of the brain by reprogramming gene expression patterns, which may result in alterations in adult behavior.
    Description
    Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Toxicological Sciences 160 (2017): 386-397, doi:10.1093/toxsci/kfx192.
    Collections
    • Biology
    Suggested Citation
    Preprint: Aluru, Neelakanteswar, Karchner, Sibel I., Glazer, Lilah, "Early life exposure to low levels of AHR agonist PCB126 (3,3’,4,4’,5- pentachlorobiphenyl) reprograms gene expression in adult brain", 2017-09, https://doi.org/10.1093/toxsci/kfx192, https://hdl.handle.net/1912/9298
     

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Tide-related changes in mRNA abundance of aromatases and estrogen receptors in the ovary and brain of the threespot wrasse Halichoeres trimaculatus 

      Oh, Dae-Ju; Hur, Sung-Pyo; Bouchekioua, Selma; Takeuchi, Yuki; Udagawa, Shingo; Aluru, Neelakanteswar; Park, Yong-Ju; Park, Ji-Gweon; Kim, Se-Jae; Moon, Thomas W.; Vijayan, Mathilakath M.; Takemura, Akihiro (2018-05)
      The threespot wrasse (Halichoeres trimaculatus; Family Labridae) is a common coral reef species of the Indo-Pacific Ocean. Given that this species spawns daily at high tide (HT), we hypothesized that endocrine changes ...
    • Thumbnail

      Expansion of voltage-dependent Na+ channel gene family in early tetrapods coincided with the emergence of terrestriality and increased brain complexity 

      Zakon, Harold H.; Jost, Manda C.; Lu, Ying (2010-11-29)
      Mammals have 10 voltage-dependent sodium (Nav) channel genes. Nav channels are expressed in different cell types with different sub-cellular distributions and are critical for many aspects of neuronal processing. The ...
    • Thumbnail

      Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic 

      Montie, Eric W.; Reddy, Christopher M.; Gebbink, Wouter A.; Touhey, Kathleen M.; Hahn, Mark E.; Letcher, Robert J. (2009-03-12)
      Concentrations of several congeners and classes of organohalogen contaminants (OHCs) and/or their metabolites, namely organochlorine pesticides (OCs), polychlorinated biphenyls (PCBs), hydroxylated-PCBs (OH-PCBs), ...
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo