Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling
Date
2016-04-22Author
Tandon, Panna
Concept link
Conlon, Frank
Concept link
Furlow, J. David
Concept link
Horb, Marko E.
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/9107As published
https://doi.org/10.1016/j.ydbio.2016.04.009DOI
10.1016/j.ydbio.2016.04.009Keyword
CRISPR-Cas; TALENs; J strain; Xenopus laevis; Xenopus tropicalis; Knock-in; Human disease modelAbstract
The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, external development, and simple housing requirements, with additional advantages of large embryos, highly conserved developmental processes, and close evolutionary relationship to higher vertebrates. There are two main species of Xenopus used in biomedical research, Xenopus laevis and Xenopus tropicalis; the common perception is that both species are excellent models for embryological and cell biological studies, but only Xenopus tropicalis is useful as a genetic model. The recent completion of the Xenopus laevis genome sequence combined with implementation of genome editing tools, such as TALENs (transcription activator-like effector nucleases) and CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated nucleases), greatly facilitates the use of both Xenopus laevis and Xenopus tropicalis for understanding gene function in development and disease. In this paper, we review recent advances made in Xenopus laevis and Xenopus tropicalis with TALENs and CRISPR-Cas and discuss the various approaches that have been used to generate knockout and knock-in animals in both species. These advances show that both Xenopus species are useful for genetic approaches and in particular counters the notion that Xenopus laevis is not amenable to genetic manipulations.
Description
© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 325-335, doi:10.1016/j.ydbio.2016.04.009.
Collections
Suggested Citation
Developmental Biology 426 (2017): 325-335The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International
Related items
Showing items related by title, author, creator and subject.
-
Xenopus as a model for GI/pancreas disease
Salanga, Matthew C.; Horb, Marko E. (2015-03)Diseases affecting endodermal organs like the pancreas, lung and gastrointestinal (GI) tract have a substantial impact on human welfare. Since many of these are congenital defects that arise as a result of defects during ... -
Repurposing human PDE4 inhibitors for neglected tropical diseases : design, synthesis and evaluation of cilomilast analogues as Trypanosoma brucei PDEB1 inhibitors
Amata, Emanuele; Bland, Nicholas D.; Hoyt, Charles T.; Settimo, Luca; Campbell, Robert K.; Pollastri, Michael P. (2014-07)A medicinal chemistry exploration of the human phosphodiesterase 4 (hPDE4) inhibitor cilomilast (1) was undertaken in order to identify inhibitors of phosphodiesterase B1 of Trypanosoma brucei (TbrPDEB1). T. brucei is the ... -
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos
Naert, Thomas; Tulkens, Dieter; Edwards, Nicole A.; Carron, Marjolein; Shaidani, Nikko-Ideen; Wlizla, Marcin; Boel, Annekatrien; Demuynck, Suzan; Horb, Marko E.; Coucke, Paul; Willaert, Andy; Zorn, Aaron M.; Vleminckx, Kris (Nature Research, 2020-09-04)CRISPR/Cas9 genome editing has revolutionized functional genomics in vertebrates. However, CRISPR/Cas9 edited F0 animals too often demonstrate variable phenotypic penetrance due to the mosaic nature of editing outcomes ...