Visualizing calcium flux in freely moving nematode embryos
Ardiel, Evan L.
Daniels, Jonathan S.
MetadataShow full item record
The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy (LSFM) to address the challenges associated with functional imaging at this developmental stage, we recorded calcium dynamics in muscles and neurons and developed analysis strategies to relate activity and movement. In muscles, we found that the initiation of twitching was associated with a spreading calcium wave in a dorsal muscle bundle. Correlated activity in muscle bundles was linked with early twitching and eventual coordinated movement. To identify neuronal correlates of behavior, we monitored brain-wide activity with subcellular resolution and identified a particularly active cell associated with muscle contractions. Finally, imaging neurons of a well-defined adult motor circuit, we found that reversals in the eggshell correlated with calcium transients in AVA interneurons.
Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Cell Press for personal use, not for redistribution. The definitive version was published in Biophysical Journal 112 (2017): 1975-1983, doi:10.1016/j.bpj.2017.02.035.
The publisher requires that this item be embargoed until 2018-05-09. Please check back after 2018-05-09.
Suggested CitationPreprint: Ardiel, Evan L., Kumar, Abhishek, Marbach, Joseph, Christensen, Ryan, Gupta, Rishi, Duncan, William, Daniels, Jonathan S., Stuurman, Nico, Colón-Ramos, Daniel, Shroff, Hari, "Visualizing calcium flux in freely moving nematode embryos", 2017-02, https://doi.org/10.1016/j.bpj.2017.02.035, https://hdl.handle.net/1912/9042
Showing items related by title, author, creator and subject.
Intentional versus unintentional nitrogen use in the United States : trends, efficiency and implications Houlton, Benjamin Z.; Boyer, Elizabeth; Finzi, Adrien C.; Galloway, James; Leach, Allison; Liptzin, Daniel; Melillo, Jerry M.; Rosenstock, Todd S.; Sobota, Daniel J.; Townsend, Alan R. (Springer, 2012-10-13)Human actions have both intentionally and unintentionally altered the global economy of nitrogen (N), with both positive and negative consequences for human health and welfare, the environment and climate change. Here we ...
Origin of the deep Bering Sea nitrate deficit : constraints from the nitrogen and oxygen isotopic composition of water column nitrate and benthic nitrate fluxes Lehmann, Moritz F.; Sigman, Daniel M.; McCorkle, Daniel C.; Brunelle, Brigitte G.; Hoffmann, Sharon S.; Kienast, Markus; Cane, Greg; Clement, Jaclyn (American Geophysical Union, 2005-10-12)On the basis of the normalization to phosphate, a significant amount of nitrate is missing from the deep Bering Sea (BS). Benthic denitrification has been suggested previously to be the dominant cause for the BS nitrate ...
Richards, Clark G.; Bourgault, Daniel; Galbraith, Peter S.; Hay, Alex; Kelley, Daniel E. (John Wiley & Sons, 2013-01-30)The shoaling of horizontally propagating internal waves may represent an important source of mixing and transport in estuaries and coastal seas. Including such effects in numerical models demands improvements in the ...