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dc.contributor.authorWlizla, Marcin  Concept link
dc.contributor.authorFalco, Rosalia  Concept link
dc.contributor.authorPeshkin, Leonid  Concept link
dc.contributor.authorParlow, Albert F.  Concept link
dc.contributor.authorHorb, Marko E.  Concept link
dc.identifier.citationDevelopmental Biology 426 (2017): 442–448en_US
dc.description© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 442–448, doi:10.1016/j.ydbio.2016.05.028.en_US
dc.description.abstractInjection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well as lack of a well tested alternative have resulted in frustrating experimental delays in laboratories that predominantly use Xenopus in their research. Mammalian Luteinizing Hormones (LH) share structural similarity, functional equivalency, and bind the same receptor as hCG; this suggests that LH may serve as a good alternative to hCG for promoting ovulation in Xenopus. LH has been found to induce maturation of Xenopus oocytes in vitro, but whether it can be used to induce ovulation in vivo has not been examined. Here we compared the ability of four mammalian LH proteins, bovine (bLH), human (hLH), ovine (oLH), porcine (pLH), to induce ovulation in Xenopus when injected into the dorsal lymph sac of sexually mature females. We find that both ovine and human LH, but not bovine or porcine, are good substitutes for hCG for induction of ovulation in WT and J strain Xenopus laevis and Xenopus tropicalis.en_US
dc.description.sponsorshipThis work was supported by a grant from the NIHP40OD010997.en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectXenopus laevisen_US
dc.subjectJ strainen_US
dc.subjectLuteinizing Hormoneen_US
dc.subjectChorionic gonadotropinen_US
dc.titleLuteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopusen_US

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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International