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dc.contributor.authorRatzan, Wil  Concept link
dc.contributor.authorFalco, Rosalia  Concept link
dc.contributor.authorSalanga, Cristy  Concept link
dc.contributor.authorSalanga, Matthew C.  Concept link
dc.contributor.authorHorb, Marko E.  Concept link
dc.date.accessioned2017-06-02T14:25:15Z
dc.date.available2017-06-02T14:25:15Z
dc.date.issued2016-03-15
dc.identifier.citationDevelopmental Biology 426 (2017): 188–193en_US
dc.identifier.urihttps://hdl.handle.net/1912/9013
dc.description© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 188–193, doi:10.1016/j.ydbio.2016.03.006.en_US
dc.description.abstractCompletion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated that TALENs are able to induce mutations in F0 Xenopus embryos, but none has demonstrated germline transmission of such mutations in X. laevis. In this report we used the oocyte host-transfer method to generate mutations in both tyrosinase homeologs and found highly-penetrant germline mutations; in contrast, embryonic injections yielded few germline mutations. We also compared the distribution of mutations in several F0 somatic tissues and germ cells and found that the majority of mutations in each tissue were different. These results establish that X. laevis J strain animals are very useful for generating germline mutations and that the oocyte host-transfer method is an efficient technique for generating mutations in both homeologs.en_US
dc.description.sponsorshipThis work was supported by grants from the NIH (OD010997 and HD084409).en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.urihttps://doi.org/10.1016/j.ydbio.2016.03.006
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectXenopus laevisen_US
dc.subjectTALENsen_US
dc.subjectJ strainen_US
dc.subjectTyrosinaseen_US
dc.subjectOocyte host-transferen_US
dc.subjectGenome editingen_US
dc.titleGeneration of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transferen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.ydbio.2016.03.006


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International