Mesodinium rubrum exhibits genus-level but not species-level cryptophyte prey selection
MetadataShow full item record
KeywordMixotrophy; Acquired phototrophy; Kleptoplastidy; Grazing; Prey selection; Species-specific qPCR; Mesodinium; Teleaulax
The marine ciliate Mesodinium rubrum is known to form large non-toxic red water blooms in estuarine and coastal upwelling regions worldwide. This ciliate relies predominantly upon photosynthesis by using plastids and other organelles it acquires from cryptophyte prey. Although M. rubrum is capable of ingesting different species of cryptophytes, mainly Teleaulax amphioxeia plastids have been detected from wild M. rubrum populations. These observations suggest that either M. rubrum is a selective feeder, or T. amphioxeia are taken up because of higher availability. To test these hypotheses, we determined whether the ciliate showed different grazing rates, growth responses, or plastid retention dynamics when offered Storeatula major, T. amphioxeia, T. acuta, or a mix. When M. rubrum was offered the cryptophyte S. major as prey, no evidence was found for ingestion. In contrast, M. rubrum grazed both Teleaulax spp. equally, was able to easily switch plastid type between them, and the ratio of each in the ciliate reflected the abundance of free-living prey in the culture. M. rubrum grew equally well when acclimated to each plastid type or when having mixed plastids. However, when offered single prey, T. amphioxeia could sustain higher M. rubrum growth rates (μ) over longer periods. Compared to other M. rubrum strains, this culture had higher grazing rates, greater ingestion requirements for reaching μmax, and appeared to rely more on plastid sequestration than de novo division of cryptophyte organelles. Our results suggest that while M. rubrum may prefer Teleaulax-like cryptophytes, they do not select among the species used here.
© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Aquatic Microbial Ecology 78 (2017):147-159, doi:10.3354/ame01809.
Suggested CitationAquatic Microbial Ecology 78 (2017):147-159
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Schmidt, Victor T.; Reveillaud, Julie; Zettler, Erik R.; Mincer, Tracy J.; Murphy, Leslie G.; Amaral-Zettler, Linda A. (Frontiers Media, 2014-11-13)The genus Vibrio is a metabolically diverse group of facultative anaerobic bacteria, common in aquatic environments and marine hosts. The genus contains several species of importance to human health and aquaculture, including ...
Schneider, Robin J.; Roe, Kelly L.; Hansel, Colleen M.; Voelker, Bettina M. (Frontiers Media, 2016-03-30)Biological production and decay of the reactive oxygen species (ROS) hydrogen peroxide (H2O2) and superoxide (O−2) likely have significant effects on the cycling of trace metals and carbon in marine systems. In this study, ...
Large-scale comparative phenotypic and genomic analyses reveal ecological preferences of Shewanella species and identify metabolic pathways conserved at the genus level Rodrigues, Jorge L. M.; Serres, Margrethe H.; Tiedje, James M. (2011-04-27)The use of comparative genomics among different microbiological species has increased substantially as sequence technologies become more affordable. However, efforts to fully link a genotype to its phenotype remain limited ...