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17β-Hydroxysteroid dehydrogenase (17β-HSD) in scleractinian corals and zooxanthellae

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dc.contributor.author Blomquist, Charles H.
dc.contributor.author Lima, P. H.
dc.contributor.author Tarrant, Ann M.
dc.contributor.author Atkinson, M. J.
dc.contributor.author Atkinson, S.
dc.date.accessioned 2006-04-19T18:43:29Z
dc.date.available 2006-04-19T18:43:29Z
dc.date.issued 2005-12-18
dc.identifier.uri http://hdl.handle.net/1912/888
dc.description Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 143 (2006): 397-403, doi:10.1016/j.cbpb.2005.12.017. en
dc.description.abstract Steroid metabolism studies have yielded evidence of 17β-hydroxysteroid dehydrogenase (17β-HSD) activity in corals. This project was undertaken to clarify whether there are multiple isoforms of 17β-HSD, whether activity levels vary seasonally, and if zooxanthellae contribute to activity. 17β-HSD activity was characterized in zooxanthellate and azooxanthellate coral fragments collected in summer and winter and in zooxanthellae cultured from M. capitata. More specifically, 17β-HSD activity was characterized with regard to steroid substrate and inhibitor specificity, coenzyme specificity, and Michaelis constants for estradiol (E2) and NADP+. Six samples each of M. capitata and T. coccinea (three summer, three winter) were assayed with E2 and NADP+. Specific activity levels (pmol/mg protein) varied 10-fold among M. capitata samples and 6-fold among T. coccinea samples. There was overlap of activity levels between summer and winter samples. NADP+/NAD+ activity ratios varied from 1.6 to 22.2 for M. capatita, 2.3 to 3.8 for T. coccinea and 0.7 to 1.1 for zooxanthellae. Coumestrol was the most inhibitory of the steroids and phytoestrogens tested. Our data confirm that corals and zooxanthellae contain 17β-HSD and are consistent with the presence of more than one isoform of the enzyme. en
dc.description.sponsorship Support for this work was provided by the EPA STAR fellowship program and the University of Hawaii Sea Grant College Program. en
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dc.language.iso en_US en
dc.relation.uri http://dx.doi.org/10.1016/j.cbpb.2005.12.017
dc.subject 17β-hydroxysteroid dehydrogenase en
dc.subject Steroid en
dc.subject Coral en
dc.subject Invertebrate en
dc.subject Zooxanthellae en
dc.subject Phytoestrogens en
dc.subject Estradiol en
dc.subject Scleractinia en
dc.title 17β-Hydroxysteroid dehydrogenase (17β-HSD) in scleractinian corals and zooxanthellae en
dc.type Preprint en


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