Transcriptomic insights into genetic diversity of protein-coding genes in X. laevis
Pearl, Esther J.
Horb, Marko E.
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We characterize the genetic diversity of Xenopus laevis strains using RNA-seq data and allele- specific analysis. This data provides a catalogue of coding variation, which can be used for improving the genomic sequence, as well as for better sequence alignment, probe design, and proteomic analysis. In addition, we paint a broad picture of the genetic landscape of the species by functionally annotating different classes of mutations with a well-established prediction tool (PolyPhen-2). Further, we specifically compare the variation in the progeny of four crosses: inbred genomic (J)- strain, outbred albino (B)-strain, and two hybrid crosses of J and B strains. We identify a subset of mutations specific to the B strain, which allows us to investigate the selection pressures affecting duplicated genes in this allotetraploid. From these crosses we find the ratio of non-synonymous to synonymous mutations is lower in duplicated genes, which suggests that they are under greater purifying selection. Surprisingly, we also find that function-altering ("damaging") mutations constitute a greater fraction of the non-synonymous variants in this group, which suggests a role for subfunctionalization in coding variation affecting duplicated genes.
© The Author(s), 2017. This is the author's version of the work and is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 424 (2017): 181-188, doi:10.1016/j.ydbio.2017.02.019
Suggested CitationPreprint: Savova, Virginia, Pearl, Esther J., Boke, Elvan, Nag, Anwesha, Adzhubei, Ivan, Horb, Marko E., Peshkin, Leonid, "Transcriptomic insights into genetic diversity of protein-coding genes in X. laevis", 2017-03, https://doi.org/10.1016/j.ydbio.2017.02.019, https://hdl.handle.net/1912/8829
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