Genome-wide transcriptomics of aging in the rotifer Brachionus manjavacas, an emerging model system
MetadataShow full item record
Understanding gene expression changes over lifespan in diverse animal species will lead to insights to conserved processes in the biology of aging and allow development of interventions to improve health. Rotifers are small aquatic invertebrates that have been used in aging studies for nearly 100 years and are now re-emerging as a modern model system. To provide a baseline to evaluate genetic responses to interventions that change health throughout lifespan and a framework for new hypotheses about the molecular genetic mechanisms of aging, we examined the transcriptome of an asexual female lineage of the rotifer Brachionus manjavacas at five life stages: eggs, neonates, and early-, late-, and post-reproductive adults. There are widespread shifts in gene expression over the lifespan of B. manjavacas; the largest change occurs between neonates and early reproductive adults and is characterized by down-regulation of developmental genes and up-regulation of genes involved in reproduction. The expression profile of post-reproductive adults was distinct from that of other life stages. While few genes were significantly differentially expressed in the late- to post-reproductive transition, gene set enrichment analysis revealed multiple down-regulated pathways in metabolism, maintenance and repair, and proteostasis, united by genes involved in mitochondrial function and oxidative phosphorylation. This study provides the first examination of changes in gene expression over lifespan in rotifers. We detected differential expression of many genes with human orthologs that are absent in Drosophila and C. elegans, highlighting the potential of the rotifer model in aging studies. Our findings suggest that small but coordinated changes in expression of many genes in pathways that integrate diverse functions drive the aging process. The observation of simultaneous declines in expression of genes in multiple pathways may have consequences for health and longevity not detected by single- or multi-gene knockdown in otherwise healthy animals. Investigation of subtle but genome-wide change in these pathways during aging is an important area for future study.
© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in BMC Genomics 18 (2017): 217, doi:10.1186/s12864-017-3540-x.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Comparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg production Hanson, Sara J.; Stelzer, Claus-Peter; Mark Welch, David B.; Logsdon, John M. (BioMed Central, 2013-06-19)Sexual reproduction is a widely studied biological process because it is critically important to the genetics, evolution, and ecology of eukaryotes. Despite decades of study on this topic, no comprehensive explanation has ...
The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP) : illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing Keeling, Patrick J.; Burki, Fabien; Wilcox, Heather M.; Allam, Bassem; Allen, Eric E.; Amaral-Zettler, Linda A.; Armbrust, E. Virginia; Archibald, John M.; Bharti, Arvind K.; Bell, Callum J.; Beszteri, Bank; Bidle, Kay D.; Cameron, Connor T.; Campbell, Lisa; Caron, David A.; Cattolico, Rose Ann; Collier, Jackie L.; Coyne, Kathryn J.; Davy, Simon K.; Deschamps, Phillipe; Dyhrman, Sonya T.; Edvardsen, Bente; Gates, Ruth D.; Gobler, Christopher J.; Greenwood, Spencer J.; Guida, Stephanie M.; Jacobi, Jennifer L.; Jakobsen, Kjetill S.; James, Erick R.; Jenkins, Bethany D.; John, Uwe; Johnson, Matthew D.; Juhl, Andrew R.; Kamp, Anja; Katz, Laura A.; Kiene, Ronald P.; Kudryavtsev, Alexander N.; Leander, Brian S.; Lin, Senjie; Lovejoy, Connie; Lynn, Denis; Marchetti, Adrian; McManus, George; Nedelcu, Aurora M.; Menden-Deuer, Susanne; Miceli, Cristina; Mock, Thomas; Montresor, Marina; Moran, Mary Ann; Murray, Shauna A.; Nadathur, Govind; Nagai, Satoshi; Ngam, Peter B.; Palenik, Brian; Pawlowski, Jan; Petroni, Giulio; Piganeau, Gwenael; Posewitz, Matthew C.; Rengefors, Karin; Romano, Giovanna; Rumpho, Mary E.; Rynearson, Tatiana A.; Schilling, Kelly B.; Schroeder, Declan C.; Simpson, Alastair G. B.; Slamovits, Claudio H.; Smith, David R.; Smith, G. Jason; Smith, Sarah R.; Sosik, Heidi M.; Stief, Peter; Theriot, Edward; Twary, Scott N.; Umale, Pooja E.; Vaulot, Daniel; Wawrik, Boris; Wheeler, Glen L.; Wilson, William H.; Xu, Yan; Zingone, Adriana; Worden, Alexandra Z. (Public Library of Science, 2014-06-24)Microbial ecology is plagued by problems of an abstract nature. Cell sizes are so small and population sizes so large that both are virtually incomprehensible. Niches are so far from our everyday experience as to make their ...
Gladyshev, Eugene A.; Arkhipova, Irina R. (2010-01)Bdelloid rotifers are microscopic invertebrate animals best known for their ancient asexuality and the ability to survive desiccation at any life stage. Both factors are expected to have a profound influence on their ...