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dc.contributor.authorShribak, Michael  Concept link
dc.contributor.authorLarkin, Kieran G.  Concept link
dc.contributor.authorBiggs, David  Concept link
dc.date.accessioned2017-01-11T19:09:35Z
dc.date.available2017-01-11T19:09:35Z
dc.date.issued2017-01-06
dc.identifier.citationJournal of Biomedical Optics 22 (2017): 016006en_US
dc.identifier.urihttps://hdl.handle.net/1912/8651
dc.descriptionAuthor Posting. © Society of Photo Optical Instrumentation Engineers, 2017. This article is posted here by permission of Society of Photo Optical Instrumentation Engineers for personal use, not for redistribution. The definitive version was published in Journal of Biomedical Optics 22 (2017): 016006, doi:10.1117/1.JBO.22.1.016006.en_US
dc.description.abstractWe describe the principles of using orientation-independent differential interference contrast (OI-DIC) microscopy for mapping optical path length (OPL). Computation of the scalar two-dimensional OPL map is based on an experimentally received map of the OPL gradient vector field. Two methods of contrast enhancement for the OPL image, which reveal hardly visible structures and organelles, are presented. The results obtained can be used for reconstruction of a volume image. We have confirmed that a standard research grade light microscope equipped with the OI-DIC and 100×/1.3 NA objective lens, which was not specially selected for minimum wavefront and polarization aberrations, provides OPL noise level of ∼0.5  nm and lateral resolution if ∼300  nm at a wavelength of 546 nm. The new technology is the next step in the development of the DIC microscopy. It can replace standard DIC prisms on existing commercial microscope systems without modification. This will allow biological researchers that already have microscopy setups to expand the performance of their systems.en_US
dc.description.sponsorshipThis publication was made possible by Grant No. R01-GM101701 from the National Institute of General Medical Sciences, National Institutes of Health.en_US
dc.language.isoen_USen_US
dc.publisherSPIEen_US
dc.relation.urihttps://doi.org/10.1117/1.JBO.22.1.016006
dc.titleMapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopyen_US
dc.typeArticleen_US
dc.identifier.doi10.1117/1.JBO.22.1.016006


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