Hyperpolarization-activated currents and subthreshold resonance in granule cells of the olfactory bulb
Ferguson, Katie A.
Meijer, Dimphna H.
Araneda, Ricardo C.
MetadataShow full item record
An important contribution to neural circuit oscillatory dynamics is the ongoing activation and inactivation of hyperpolarization-activated currents (Ih). Network synchrony dynamics play an important role in the initial processing of odor signals by the main olfactory bulb (MOB) and accessory olfactory bulb (AOB). In the mouse olfactory bulb, we show that Ih is present in granule cells (GCs), the most prominent inhibitory neuron in the olfactory bulb, and that Ih underlies subthreshold resonance in GCs. In accord with the properties of Ih, the currents exhibited sensitivity to changes in extracellular K concentration and ZD7288 (4-ethylphenylamino-1,2-dimethyl-6-methylaminopyrimidin chloride), a blocker of Ih. ZD7288 also caused GCs to hyperpolarize and increase their input resistance, suggesting that Ih is active at rest in GCs. The inclusion of cAMP in the intracellular solution shifted the activation of Ih to less negative potentials in the MOB, but not in the AOB, suggesting that channels with different subunit composition mediate Ih in these regions. Furthermore, we show that mature GCs exhibit Ih-dependent subthreshold resonance in the theta frequency range (4–12 Hz). Another inhibitory subtype in the MOB, the periglomerular cells, exhibited Ih-dependent subthreshold resonance in the delta range (1–4 Hz), while principal neurons, the mitral cells, do not exhibit Ih-dependent subthreshold resonance. Importantly, Ih size, as well as the strength and frequency of resonance in GCs, exhibited a postnatal developmental progression, suggesting that this development of Ih in GCs may differentially contribute to their integration of sensory input and contribution to oscillatory circuit dynamics.
© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in eNeuro 3 (2016): e0197-16.2016, doi:10.1523/ENEURO.0197-16.2016.
Suggested CitationArticle: Hu, Ruilong, Ferguson, Katie A., Whiteus, Christina, Meijer, Dimphna H., Araneda, Ricardo C., "Hyperpolarization-activated currents and subthreshold resonance in granule cells of the olfactory bulb", eNeuro 3 (2016): e0197-16.2016, DOI:10.1523/ENEURO.0197-16.2016, https://hdl.handle.net/1912/8542
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Lvov, Yuri V.; Polzin, Kurt L.; Yokoyama, Naoto (American Meteorological Society, 2012-05)The spectral energy density of the internal waves in the open ocean is considered. The Garrett and Munk spectrum and the resonant kinetic equation are used as the main tools of the study. Evaluations of a resonant kinetic ...
Homma, Ryota; Kovalchuk, Yuri; Konnerth, A.; Cohen, Lawrence B.; Garaschuk, Olga (Frontiers Media S.A., 2013-02-21)Juxtaglomerular neurons represent one of the largest cellular populations in the mammalian olfactory bulb yet their role for signal processing remains unclear. We used two-photon imaging and electrophysiological recordings ...
Braubach, Oliver R.; Tombaz, Tuce; Geiller, Tristan; Homma, Ryota; Bozza, Thomas; Cohen, Lawrence B.; Choi, Yunsook (Nature Publishing Group, 2018-10-08)Glomeruli are the functional units of olfactory information processing but little remains known about their individual unit function. This is due to their widespread activation by odor stimuli. We expressed channelrhodopsin-2 ...