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dc.contributor.authorMoriwaki, Takashi  Concept link
dc.contributor.authorGoshima, Gohta  Concept link
dc.date.accessioned2016-11-09T20:58:09Z
dc.date.available2017-05-02T17:43:15Z
dc.date.issued2016-10-31
dc.identifier.citationJournal of Cell Biology 215 (2016): 357en_US
dc.identifier.urihttps://hdl.handle.net/1912/8506
dc.description© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 215 (2016): 357, doi:10.1083/jcb.201604118.en_US
dc.description.abstractCytoplasmic microtubules (MTs) undergo growth, shrinkage, and pausing. However, how MT polymerization cycles are produced and spatiotemporally regulated at a molecular level is unclear, as the entire cycle has not been recapitulated in vitro with defined components. In this study, we reconstituted dynamic MT plus end behavior involving all three phases by mixing tubulin with five Drosophila melanogaster proteins (EB1, XMAP215Msps, Sentin, kinesin-13Klp10A, and CLASPMast/Orbit). When singly mixed with tubulin, CLASPMast/Orbit strongly inhibited MT catastrophe and reduced the growth rate. However, in the presence of the other four factors, CLASPMast/Orbit acted as an inducer of pausing. The mitotic kinase Plk1Polo modulated the activity of CLASPMast/Orbit and kinesin-13Klp10A and increased the dynamic instability of MTs, reminiscent of mitotic cells. These results suggest that five conserved proteins constitute the core factors for creating dynamic MTs in cells and that Plk1-dependent phosphorylation is a crucial event for switching from the interphase to mitotic mode.en_US
dc.description.sponsorshipThis work was supported by the Grants-in-Aid for Scientific Research (Japan Society for the Promotion of Science KAK ENHI; 15H01317 and 15KT0077).en_US
dc.language.isoen_USen_US
dc.publisherRockefeller University Pressen_US
dc.relation.urihttps://doi.org/10.1083/jcb.201604118
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/
dc.titleFive factors can reconstitute all three phases of microtubule polymerization dynamicsen_US
dc.typeArticleen_US
dc.description.embargo2017-04-30en_US
dc.identifier.doi10.1083/jcb.201604118


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Attribution-NonCommercial-ShareAlike 3.0 Unported
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 Unported