Cytochrome P450 induced differentially in endothelial cells cultured from different organs of Anguilla rostrata
Citable URI
https://hdl.handle.net/1912/845As published
https://doi.org/10.1290/0409063.1Keyword
Teleost; Eel; Fish; Microvasculature; Endothelium; pHAH; Cytochrome P450; EROD; DioxinAbstract
Endothelial cells are a structural barrier and an active regulator of many bodily
processes. CYP1A activity is induced in the endothelium of teleosts and mammals
exposed to lipophilic xenobiotics, such as polycyclic aromatic hydrocarbons, and can
have significant consequences for endothelial functions. We exposed cultures of
characterized endothelial cells from the heart, kidney and rete mirabile of the eel,
Anguilla rostrata, to AhR agonists. In heart endothelial cells the maximum response
(based on EROD activity) to TCDD, 113 pmol/mg-min, was at 1 nM TCDD and the peak
response to βNF, 135 pmol/mg-min, was at 3 μM βNF. The maximum response to
TCDD in the kidney endothelial cells is 12 pmol/mg-min at 0.3 nM TCDD. The rete
mirabile capillary endothelial cells responded minimally or not at all to exposure to
TCDD and βNF. Both the heart and kidney endothelial cells (but not the rete mirabile
capillary cells) have a low level of EROD activity (12.7 and 5.2 pmol/mg-min
respectively) in untreated or DMSO-treated cells. The robust response of the heart
endothelial cells to induction and the lack of response in the rete mirabile capillary
endothelial cells indicate that these cells are a good resource to use to investigate the
physiological consequences of AhR agonist exposure and CYP1A induction in different
areas of the vasculature.
Description
Author Posting. © The Authors, 2004. This is the author's version of the work. It is posted here by permission of Society for In Vitro Biology for personal use, not for redistribution. The definitive version was published in In Vitro Cellular & Developmental Biology - Animal 41 (2005): 57-63, doi:10.1290/0409063.1.
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Suggested Citation
Preprint: Garrick, Rita Anne, Woodin, Bruce R., Stegeman, John J., "Cytochrome P450 induced differentially in endothelial cells cultured from different organs of Anguilla rostrata", 2004-12-15, https://doi.org/10.1290/0409063.1, https://hdl.handle.net/1912/845Related items
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