Cytochrome P450 induced differentially in endothelial cells cultured from different organs of Anguilla rostrata
MetadataShow full item record
Endothelial cells are a structural barrier and an active regulator of many bodily processes. CYP1A activity is induced in the endothelium of teleosts and mammals exposed to lipophilic xenobiotics, such as polycyclic aromatic hydrocarbons, and can have significant consequences for endothelial functions. We exposed cultures of characterized endothelial cells from the heart, kidney and rete mirabile of the eel, Anguilla rostrata, to AhR agonists. In heart endothelial cells the maximum response (based on EROD activity) to TCDD, 113 pmol/mg-min, was at 1 nM TCDD and the peak response to βNF, 135 pmol/mg-min, was at 3 μM βNF. The maximum response to TCDD in the kidney endothelial cells is 12 pmol/mg-min at 0.3 nM TCDD. The rete mirabile capillary endothelial cells responded minimally or not at all to exposure to TCDD and βNF. Both the heart and kidney endothelial cells (but not the rete mirabile capillary cells) have a low level of EROD activity (12.7 and 5.2 pmol/mg-min respectively) in untreated or DMSO-treated cells. The robust response of the heart endothelial cells to induction and the lack of response in the rete mirabile capillary endothelial cells indicate that these cells are a good resource to use to investigate the physiological consequences of AhR agonist exposure and CYP1A induction in different areas of the vasculature.
Author Posting. © The Authors, 2004. This is the author's version of the work. It is posted here by permission of Society for In Vitro Biology for personal use, not for redistribution. The definitive version was published in In Vitro Cellular & Developmental Biology - Animal 41 (2005): 57-63, doi:10.1290/0409063.1.
Showing items related by title, author, creator and subject.
Involvement of Cytochrome P450 1A in the toxicity of aryl hydrocarbon receptor agonists : alteration arachidonic acid metabolism and production of reactive oxygen species Schlezinger, Jennifer J. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1998-08)Two cytochrome P4501A-dependent mechanisms of aryl hydrocarbon receptor (AhR) agonist toxicity were examined in the marine teleost scup (Stenotomus chrysops), alteration of arachidonic acid (AA) metabolism and production ...
Diversity and characterization of novel Cytochrome P450 2 genes in the marine teleost Fundulus Heteroclitus Oleksiak, Marjorie F. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1997-09)The multiplicity and roles of cytochrome P450 CYP genes in the 2 family are not well known in fish. Characterizations of CYP2 genes were done in the killifish, Fundulus heteroclitus. Multiple cDNAs from three CYP2 ...
Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops Klotz, Alan V. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1983-08)Three cytochrome P-450 forms were highly purified (8-12 nmol/mg) from the hepatic microsomes of the untreated coastal marine fish Stenotomus chrysops (scup) by detergent solubilization and column chromatography. Scup ...