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    Cytochrome P4501A is induced in endothelial cell lines from the kidney and lung of the bottlenose dolphin, Tursiops truncatus

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    GarrickAQTOX-D-05e.pdf (830.3Kb)
    Date
    2005-10-15
    Author
    Garrick, Rita Anne  Concept link
    Woodin, Bruce R.  Concept link
    Wilson, Joanna Y.  Concept link
    Middlebrooks, Bobby L.  Concept link
    Stegeman, John J.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/844
    As published
    https://doi.org/10.1016/j.aquatox.2005.10.005
    Keyword
     Endothelium; CYP1A; EROD; Dioxin; Cetacean; Microvascular 
    Abstract
    Marine mammals respond to the presence of polycyclic and planar halogenated aromatic hydrocarbons (PAH or PHAH) with the induced expression in endothelium of cytochrome P4501A1, regulated through the aryl hydrocarbon receptor (AHR) transcription factor. Physiological responses in other animals, such as edema and inflammation indicate that the endothelium may be compromised by exposure to AHR agonists, which are ubiquitous in the marine environment. In other mammals and fish the cellular and molecular consequences of exposure to AHR agonists have been elucidated in cultured endothelial cells. We have cultured and characterized cetacean endothelial cells (EC) and used them in induction studies. Endothelial cells were cultured from the lung and kidney of the bottlenose dolphin Tursiops truncatus and exposed to the AHR agonists β-naphthoflavone (βNF) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). βNF (1-3 μM) induced significant increases in CYP1A1(O-deethylation of 7-ethoxyresorufin to resorufin;EROD) activity to 3.6 and 0.92 pmol/mg/min in lung and kidney EC, respectively. TCDD was more potent than βNF, and more efficacious, with maximum induction of CYP1A1activity of 10.1 and 15.2 pmol/mg/min in lung and kidney EC at 3-10 nM TCDD. The differential response indicates that the lung and kidney endothelial cells in culture retain the ability to respond in a selective manner to specific stimuli. Both the molecular mechanisms of induction and the physiological consequences, especially in the vasculature, of toxicant exposure can be studied in this system.
    Description
    Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Aquatic Toxicology 76 (2006): 295-305, doi:10.1016/j.aquatox.2005.10.005.
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    Suggested Citation
    Preprint: Garrick, Rita Anne, Woodin, Bruce R., Wilson, Joanna Y., Middlebrooks, Bobby L., Stegeman, John J., "Cytochrome P4501A is induced in endothelial cell lines from the kidney and lung of the bottlenose dolphin, Tursiops truncatus", 2005-10-15, https://doi.org/10.1016/j.aquatox.2005.10.005, https://hdl.handle.net/1912/844
     
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