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dc.contributor.authorNunez, Illyce  Concept link
dc.contributor.authorRodriguez Pino, Marbelys  Concept link
dc.contributor.authorWiley, David J.  Concept link
dc.contributor.authorDas, Maitreyi E.  Concept link
dc.contributor.authorChen, Chuan  Concept link
dc.contributor.authorGoshima, Tetsuya  Concept link
dc.contributor.authorKume, Kazunori  Concept link
dc.contributor.authorHirata, Dai  Concept link
dc.contributor.authorToda, Takashi  Concept link
dc.contributor.authorVerde, Fulvia  Concept link
dc.identifier.citationeLife 5 (2016): e14216en_US
dc.description© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in eLife 5 (2016): e14216, doi:10.7554/eLife.14216.en_US
dc.description.abstractRNA-binding proteins contribute to the formation of ribonucleoprotein (RNP) granules by phase transition, but regulatory mechanisms are not fully understood. Conserved fission yeast NDR (Nuclear Dbf2-Related) kinase Orb6 governs cell morphogenesis in part by spatially controlling Cdc42 GTPase. Here we describe a novel, independent function for Orb6 kinase in negatively regulating the recruitment of RNA-binding protein Sts5 into RNPs to promote polarized cell growth. We find that Orb6 kinase inhibits Sts5 recruitment into granules, its association with processing (P) bodies, and degradation of Sts5-bound mRNAs by promoting Sts5 interaction with 14-3-3 protein Rad24. Many Sts5-bound mRNAs encode essential factors for polarized cell growth, and Orb6 kinase spatially and temporally controls the extent of Sts5 granule formation. Disruption of this control system affects cell morphology and alters the pattern of polarized cell growth, revealing a role for Orb6 kinase in the spatial control of translational repression that enables normal cell morphogenesis.en_US
dc.description.sponsorshipWork in FV’s laboratory is supported by the National Institutes of Health R01 grant number GM095867. Part of this work was also supported by NSF grant 0745129. TT was supported by Japan Society for the Promotion of Science grants 16H02503 and 16K14672 and by Cancer Research UK.en_US
dc.rightsAttribution 4.0 International*
dc.titleSpatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5en_US

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Except where otherwise noted, this item's license is described as Attribution 4.0 International