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dc.contributor.authorTopcuoglu, Begum D.  Concept link
dc.contributor.authorStewart, Lucy C.  Concept link
dc.contributor.authorMorrison, Hilary G.  Concept link
dc.contributor.authorButterfield, David A.  Concept link
dc.contributor.authorHuber, Julie A.  Concept link
dc.contributor.authorHolden, James F.  Concept link
dc.date.accessioned2016-09-14T17:49:29Z
dc.date.available2016-09-14T17:49:29Z
dc.date.issued2016-08-05
dc.identifier.citationFrontiers in Microbiology 7 (2016): 1240en_US
dc.identifier.urihttps://hdl.handle.net/1912/8342
dc.description© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 7 (2016): 1240, doi:10.3389/fmicb.2016.01240.en_US
dc.description.abstractThermophilic methanogens are common autotrophs at hydrothermal vents, but their growth constraints and dependence on H2 syntrophy in situ are poorly understood. Between 2012 and 2015, methanogens and H2-producing heterotrophs were detected by growth at 80∘C and 55∘C at most diffuse (7–40∘C) hydrothermal vent sites at Axial Seamount. Microcosm incubations of diffuse hydrothermal fluids at 80∘C and 55∘C demonstrated that growth of thermophilic and hyperthermophilic methanogens is primarily limited by H2 availability. Amendment of microcosms with NH4+ generally had no effect on CH4 production. However, annual variations in abundance and CH4 production were observed in relation to the eruption cycle of the seamount. Microcosm incubations of hydrothermal fluids at 80∘C and 55∘C supplemented with tryptone and no added H2 showed CH4 production indicating the capacity in situ for methanogenic H2 syntrophy. 16S rRNA genes were found in 80∘C microcosms from H2-producing archaea and H2-consuming methanogens, but not for any bacteria. In 55∘C microcosms, sequences were found from H2-producing bacteria and H2-consuming methanogens and sulfate-reducing bacteria. A co-culture of representative organisms showed that Thermococcus paralvinellae supported the syntrophic growth of Methanocaldococcus bathoardescens at 82∘C and Methanothermococcus sp. strain BW11 at 60∘C. The results demonstrate that modeling of subseafloor methanogenesis should focus primarily on H2 availability and temperature, and that thermophilic H2 syntrophy can support methanogenesis within natural microbial assemblages and may be an important energy source for thermophilic autotrophs in marine geothermal environments.en_US
dc.description.sponsorshipThis work was funded by the Gordon and Betty Moore Foundation grant GBMF 3297, the NASA Earth and Space Science Fellowship Program grant NNX11AP78H, the National Science Foundation grant OCE-1547004, with funding from NOAA/PMEL, contribution #4493, and JISAO under NOAA Cooperative Agreement NA15OAR4320063, contribution #2706.en_US
dc.language.isoen_USen_US
dc.publisherFrontiers Mediaen_US
dc.relation.urihttps://doi.org/10.3389/fmicb.2016.01240
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleHydrogen limitation and syntrophic growth among natural assemblages of thermophilic methanogens at deep-sea hydrothermal ventsen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2016.01240


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International