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dc.contributor.authorBecker, Kevin W.  Concept link
dc.contributor.authorElling, Felix J.  Concept link
dc.contributor.authorYoshinaga, Marcos Y.  Concept link
dc.contributor.authorSollinger, Andrea  Concept link
dc.contributor.authorUrich, Tim  Concept link
dc.contributor.authorHinrichs, Kai-Uwe  Concept link
dc.date.accessioned2016-08-12T17:11:39Z
dc.date.available2016-11-13T09:51:01Z
dc.date.issued2016-05
dc.identifier.urihttps://hdl.handle.net/1912/8241
dc.descriptionAuthor Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 82 (2016): 4505-4516, doi:10.1128/AEM.00772-16.en_US
dc.description.abstractA new clade of archaea has recently been proposed to constitute the seventh methanogenic order, the Methanomassiliicoccales, which is related to the Thermoplasmatales and the uncultivated archaeal clades deep-sea hydrothermal vent Euryarchaeota group 2 and marine group II Euryarchaeota but only distantly related to other methanogens. In this study, we investigated the membrane lipid composition of Methanomassiliicoccus luminyensis, the sole cultured representative of this seventh order. The lipid inventory of M. luminyensis comprises a unique assemblage of novel lipids as well as lipids otherwise typical for thermophilic, methanogenic, or halophilic archaea. For instance, glycerol sesterpanyl-phytanyl diether core lipids found mainly in halophilic archaea were detected, and so were compounds bearing either heptose or methoxylated glycosidic head groups, neither of which have been reported so far for other archaea. The absence of quinones or methanophenazines is consistent with a biochemistry of methanogenesis different from that of the methanophenazine-containing methylotrophic methanogens. The most distinctive characteristic of the membrane lipid composition of M. luminyensis, however, is the presence of tetraether lipids in which one glycerol backbone is replaced by either butane- or pentanetriol, i.e., lipids recently discovered in marine sediments. Butanetriol dibiphytanyl glycerol tetraether (BDGT) constitutes the most abundant core lipid type (>50% relative abundance) in M. luminyensis. We have thus identified a source for these unusual orphan lipids. The complementary analysis of diverse marine sediment samples showed that BDGTs are widespread in anoxic layers, suggesting an environmental significance of Methanomassiliicoccales and/or related BDGT producers beyond gastrointestinal tracts.en_US
dc.description.sponsorshipThis study was funded by the European Research Council under the European Union's Seventh Framework Programme “Ideas” Specific Programme, ERC grant agreement no. 247153 (Advanced Grant DARCLIFE; principal investigator, K.-U.H.), and by the Deutsche Forschungsgemeinschaft through the Gottfried Wilhelm Leibniz Prize awarded to K.-U.H. (Hi 616-14-1) and instrument grant Inst 144/300-1 (LC-qToF system). A.S. and T.U. were financially supported by a UNI:DOCS fellowship and a short-term travel grant (KWA) from the University of Vienna (Austria).en_US
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1128/AEM.00772-16
dc.titleUnusual butane- and pentanetriol-based tetraether lipids in Methanomassiliicoccus luminyensis, a representative of the seventh order of methanogensen_US
dc.typePreprinten_US
dc.description.embargo2016-11-13en_US


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