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    Squid giant axon contains neurofilament protein mRNA but does not synthesize neurofilament proteins

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    Date
    2016-05-05
    Author
    Gainer, Harold  Concept link
    House, Shirley  Concept link
    Kim, Dong Sun  Concept link
    Chin, Hemin  Concept link
    Pant, Harish C.  Concept link
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    Citable URI
    https://hdl.handle.net/1912/8033
    As published
    https://doi.org/10.1007/s10571-016-0382-z
    Keyword
     Squid axon; Neurofilament protein; Local protein synthesis; mRNA; Ribonuclease Protection; Schwann cell 
    Abstract
    When isolated squid giant axons are incubated in radioactive amino acids, abundant newly synthesized proteins are found in the axoplasm. These proteins are translated in the adaxonal Schwann cells and subsequently transferred into the giant axon. The question as to whether any de novo protein synthesis occurs in the giant axon itself is difficult to resolve because the small contribution of the proteins possibly synthesized intra-axonally is not easily distinguished from the large amounts of the proteins being supplied from the Schwann cells. In this paper we reexamine this issue by studying the synthesis of endogenous neurofilament (NF) proteins in the axon. Our laboratory previously showed that NF mRNA and protein is present in the squid giant axon, but not in the surrounding adaxonal glia. Therefore, if the isolated squid axon could be shown to contain newly synthesized NF protein de novo, it could not arise from the adaxonal glia. The results of experiments in this paper show that abundant 3H-labeled NF protein is synthesized in the squid giant fiber lobe containing the giant axon’s neuronal cell bodies, but despite the presence of NF mRNA in the giant axon, no labeled NF protein is detected in the giant axon. This lends support to the Glia-Axon Protein Transfer Hypothesis which posits that the squid giant axon obtains newly synthesized protein by Schwann cell transfer and not through intra-axonal protein synthesis, and further suggests that the NF mRNA in the axon is in a translationally repressed state.
    Description
    Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Cellular and Molecular Neurobiology 37 (2017): 475-486, doi:10.1007/s10571-016-0382-z.
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    • National Institute of Neurological Disorders and Stroke (NINDS) at the MBL
    Suggested Citation
    Preprint: Gainer, Harold, House, Shirley, Kim, Dong Sun, Chin, Hemin, Pant, Harish C., "Squid giant axon contains neurofilament protein mRNA but does not synthesize neurofilament proteins", 2016-05-05, https://doi.org/10.1007/s10571-016-0382-z, https://hdl.handle.net/1912/8033
     

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