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dc.contributor.authorZheng, Ning  Concept link
dc.contributor.authorJeyifous, Okunola  Concept link
dc.contributor.authorMunro, Charlotte  Concept link
dc.contributor.authorMontgomery, Johanna M.  Concept link
dc.contributor.authorGreen, William N.  Concept link
dc.identifier.citationeLife 4 (2015): e06878en_US
dc.description© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in eLife 4 (2015): e06878, doi:10.7554/eLife.06878.en_US
dc.description.abstractChanges in glutamatergic synaptic strength in brain are dependent on AMPA-type glutamate receptor (AMPAR) recycling, which is assumed to occur through a single local pathway. In this study, we present evidence that AMPAR recycling occurs through different pathways regulated by synaptic activity. Without synaptic stimulation, most AMPARs recycled in dynamin-independent endosomes containing the GTPase, Arf6. Few AMPARs recycled in dynamin-dependent endosomes labeled by transferrin receptors (TfRs). AMPAR recycling was blocked by alterations in the GTPase, TC10, which co-localized with Arf6 endosomes. TC10 mutants that reduced AMPAR recycling had no effect on increased AMPAR levels with long-term potentiation (LTP) and little effect on decreased AMPAR levels with long-term depression. However, internalized AMPAR levels in TfR-containing recycling endosomes increased after LTP, indicating increased AMPAR recycling through the dynamin-dependent pathway with synaptic plasticity. LTP-induced AMPAR endocytosis is inconsistent with local recycling as a source of increased surface receptors, suggesting AMPARs are trafficked from other sites.en_US
dc.description.sponsorshipThis work was supported by the U.S. National Institutes of Health under grant numbers NS043782, NS090903 and DA035430 (WNG). This project was also supported by the University of Chicago, Department of Neurobiology Erma Smith Scholarship from the Physiology Endowment Fund and faculty fellowships to WNG and JMM from the Marine Biological Laboratory.en_US
dc.publishereLife Sciences Publicationsen_US
dc.rightsAttribution 4.0 International*
dc.titleSynaptic activity regulates AMPA receptor trafficking through different recycling pathwaysen_US

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Except where otherwise noted, this item's license is described as Attribution 4.0 International