Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton

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2016-04-04Author
Bridges, Andrew A.
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Jentzsch, Maximilian S.
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Oakes, Patrick W.
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Occhipinti, Patricia
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Gladfelter, Amy S.
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https://hdl.handle.net/1912/7950As published
https://doi.org/10.1083/jcb.201512029DOI
10.1083/jcb.201512029Abstract
Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape.
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© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 213 (2016): 23-32, doi: 10.1083/jcb.201512029.
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Journal of Cell Biology 213 (2016): 23-32The following license files are associated with this item: