Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton
Bridges, Andrew A.
Jentzsch, Maximilian S.
Oakes, Patrick W.
Gladfelter, Amy S.
MetadataShow full item record
Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape.
© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 213 (2016): 23-32, doi: 10.1083/jcb.201512029.
Suggested CitationArticle: Bridges, Andrew A., Jentzsch, Maximilian S., Oakes, Patrick W., Occhipinti, Patricia, Gladfelter, Amy S., "Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton", Journal of Cell Biology 213 (2016): 23-32, DOI:10.1083/jcb.201512029, https://hdl.handle.net/1912/7950
The following license files are associated with this item: