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    Delayed effects of developmental exposure to low levels of the aryl hydrocarbon receptor agonist 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) on adult zebrafish behavior

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    Glazer et al_2015_Neurotox_final_2.pdf (1.263Mb)
    Date
    2015-11
    Author
    Glazer, Lilah  Concept link
    Hahn, Mark E.  Concept link
    Aluru, Neelakanteswar  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/7680
    As published
    https://doi.org/10.1016/j.neuro.2015.11.012
    Keyword
     Zebrafish; PCB126; Dioxin; Developmental neurotoxicity; Anxiety; Habituation 
    Abstract
    Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants. The most toxic PCBs are the non-ortho-substituted ("dioxin-like") congeners that act through the aryl hydrocarbon receptor (AHR) pathway. In humans, perinatal exposure to dioxin-like PCBs is associated with neurodevelopmental toxicity in children. Yet, the full potential for later-life neurobehavioral effects that result from early-life low level exposure to dioxin-like PCBs is not well understood. The objective of this study was to determine the effects of developmental exposure to low levels of dioxin-like PCBs on early- and later-life behavioral phenotypes using zebrafish as a model system. We exposed zebrafish embryos to either vehicle (DMSO) or low concentrations of PCB126 (0.3, 0.6, 1.2 nM) for 20 hours (4-24 hours post fertilization), and then reared them to adulthood in clean water. Locomotor activity was tested at two larval stages (7 and 14 days post fertilization). Adult fish were tested for anxiety-related behavior using the novel tank and shoaling assays. Adult behavioral assays were repeated several times on the same group of fish and effects on intra- and inter-trial habituation were determined. While there was no effect of PCB126 on larval locomotor activity in response to changes in light conditions, developmental exposure to PCB126 resulted in impaired short- and long-term habituation to a novel environment in adult zebrafish. Cyp1a induction was measured as an indicator for AHR activation. Despite high induction at early stages, cyp1a expression was not induced in the brains of developmentally exposed adult fish that showed altered behavior, suggesting that AHR was not activated at this stage. Our results demonstrate the effectiveness of the zebrafish model in detecting subtle and delayed behavioral effects resulting from developmental exposure to an environmental contaminant.
    Description
    Author Posting. © The Author(s), 2015. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in NeuroToxicology 52 (2016): 134-143, doi:10.1016/j.neuro.2015.11.012.
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    Suggested Citation
    Preprint: Glazer, Lilah, Hahn, Mark E., Aluru, Neelakanteswar, "Delayed effects of developmental exposure to low levels of the aryl hydrocarbon receptor agonist 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) on adult zebrafish behavior", 2015-11, https://doi.org/10.1016/j.neuro.2015.11.012, https://hdl.handle.net/1912/7680
     

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