Evolution of the chordate regeneration blastema : differential gene expression and conserved role of notch signaling during siphon regeneration in the ascidian Ciona
Byerly, Mardi S.
Jeffery, William R.
MetadataShow full item record
The regeneration of the oral siphon (OS) and other distal structures in the ascidian Ciona intestinalis occurs by epimorphosis involving the formation of a blastema of proliferating cells. Despite the longstanding use of Ciona as a model in molecular developmental biology, regeneration in this system has not been previously explored by molecular analysis. Here we have employed microarray analysis and quantitative real time RT-PCR to identify genes with differential expression profiles during OS regeneration. The majority of differentially expressed genes were downregulated during OS regeneration, suggesting roles in normal growth and homeostasis. However, a subset of differentially expressed genes was upregulated in the regenerating OS, suggesting functional roles during regeneration. Among the upregulated genes were key members of the Notch signaling pathway, including those encoding the delta and jagged ligands, two fringe modulators, and to a lesser extent the notch receptor. In situ hybridization showed a complementary pattern of delta1 and notch gene expression in the blastema of the regenerating OS. Chemical inhibition of the Notch signaling pathway reduced the levels of cell proliferation in the branchial sac, a stem cell niche that contributes progenitor cells to the regenerating OS, and in the OS regeneration blastema, where siphon muscle fibers eventually re-differentiate. Chemical inhibition also prevented the replacement of oral siphon pigment organs, sensory receptors rimming the entrance of the OS, and siphon muscle fibers, but had no effects on the formation of the wound epidermis. Since Notch signaling is involved in the maintenance of proliferative activity in both the Ciona and vertebrate regeneration blastema, the results suggest a conserved evolutionary role of this signaling pathway in chordate regeneration. The genes identified in this investigation provide the foundation for future molecular analysis of OS regeneration.
Author Posting. © The Author(s), 2015. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Developmental Biology 405 (2015): 304-315, doi:10.1016/j.ydbio.2015.07.017.
Suggested CitationPreprint: Hamada, Mayuko, Goricki, Spela, Byerly, Mardi S., Satoh, Noriyuki, Jeffery, William R., "Evolution of the chordate regeneration blastema : differential gene expression and conserved role of notch signaling during siphon regeneration in the ascidian Ciona", 2015-06, https://doi.org/10.1016/j.ydbio.2015.07.017, https://hdl.handle.net/1912/7544
Showing items related by title, author, creator and subject.
Auger, Helene; Sasakura, Yasunori; Joly, Jean-Stephane; Jeffery, William R. (2009-12-06)Ascidians have powerful capacities for regeneration but the underlying mechanisms are poorly understood. Here we examine oral siphon regeneration in the solitary ascidian Ciona intestinalis. Following amputation, the ...
Jeffery, William R. (2015-04)Regeneration studies in the tunicate Ciona intestinalis have recently been focused on the potential of adult stem cells to replace injured tissues and organs during the adult life cycle using the oral siphon (OS) as a ...
The role of regenerated nitrogen for rocky shore productivity, Cape Flattery, Washington, 2010 & 2011 Pather, Santhiska; Pfister, Catherine A.; Altabet, Mark A.; Post, David (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: firstname.lastname@example.org, 2014-02-04)Stable isotope tracers were added to exposed tidepools utilizing them as temporary mesocosms to quantify N transformation rates (Pather et al.,L&O). Large tracer signals were observed over the typical 4-5 hr experimental ...