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dc.contributor.authorKingston, Alexandra C. N.  Concept link
dc.contributor.authorKuzirian, Alan M.  Concept link
dc.contributor.authorHanlon, Roger T.  Concept link
dc.contributor.authorCronin, Thomas W.  Concept link
dc.date.accessioned2015-06-23T14:08:34Z
dc.date.available2016-05-15T07:51:17Z
dc.date.issued2015-04
dc.identifier.urihttps://hdl.handle.net/1912/7361
dc.descriptionAuthor Posting. © The Author(s), 2015. This is the author's version of the work. It is posted here by permission of The Company of Biologists for personal use, not for redistribution. The definitive version was published in Journal of Experimental Biology 218 (2015): 1596-1602, doi: 10.1242/​jeb.117945.en_US
dc.description.abstractCephalopod molluscs are renowned for their colorful and dynamic body patterns, produced by an assemblage of skin components that interact with light. These may include iridophores, leucophores, chromatophores, and (in some species) photophores. Here, we present molecular evidence suggesting that cephalopod chromatophores, small dermal pigmentary organs that reflect various colors of light, are photosensitive. RT-PCR revealed the presence of transcripts encoding rhodopsin and retinochrome within the retinas and skin of the squid Doryteuthis pealeii, and the cuttlefish Sepia officinalis and Sepia latimanus. In D. pealeii, Gqα and squid TRP channel transcripts were present in the retina and in all dermal samples. Rhodopsin, retinochrome, and Gqα transcripts were also found in RNA extracts from dissociated chromatophores isolated from D. pealeii dermal tissues. In D. pealeii, immunohistochemical staining labeled rhodopsin, retinochrome, and Gqα proteins in several chromatophore components, including pigment cell membranes, radial muscle fibers, and sheath cells. This is the first evidence that cephalopod dermal tissues, and specifically chromatophores, may possess the requisite combination of molecules required to respond to light.en_US
dc.description.sponsorshipThis research was supported by the Office of Naval Research Basic Research Challenge [grant number N00014-10-0989] via a subcontract to T.W.C. and R.T.H.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1242/​jeb.117945
dc.subjectRhodopsinen_US
dc.subjectRetinochromeen_US
dc.subjectExtraocular photoreceptoren_US
dc.subjectSkinen_US
dc.titleVisual phototransduction components in cephalopod chromatophores suggest dermal photoreceptionen_US
dc.typePreprinten_US
dc.description.embargo2016-05-15en_US


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