Ecological functions of uncultured microorganisms in the cobalt-rich ferromanganese crust of a seamount in the central Pacific are elucidated by fosmid sequencing
Post, Anton F.
MetadataShow full item record
Cobalt-rich ferromanganese is an important seafloor mineral and is abundantly present in the seamount crusts. Such crusts form potential hotspots for biogeochemical activity and microbial diversity, yet our understanding of their microbial communities is lacking. In this study, we used a cultivation-independent approach to recover genomic information and derive ecological functions of the microbes in a sediment sample collected from the cobalt-rich ferromanganese crust of a seamount region in the central Pacific. A total of 78 distinct clones were obtained by fosmid library screening with a 16S rRNA based PCR method. Proteobacteria and MGI Thaumarchaeota dominated the bacterial and archaeal 16S rRNA gene sequence results in the microbial community. Nine fosmid clones were sequenced and annotated. Numerous genes encoding proteins involved in metabolic functions and heavy metal resistance were identified, suggesting alternative metabolic pathways and stress responses that are essential for microbial survival in the cobalt-rich ferromanganese crust. In addition, genes that participate in the synthesis of organic acids and exoploymers were discovered. Reconstruction of the metabolic pathways revealed that the nitrogen cycle is an important biogeochemical process in the cobalt-rich ferromanganese crust. In addition, horizontal gene transfer (HGT) events have been observed, and most of them came from bacteria, with some occurring in archaea and plants. Clone W4-93a, belonging to MGI Thaumarchaeota, contained a region of gene synteny. Comparative analyses suggested that a high frequency of HGT events as well as genomic divergence play important roles in the microbial adaption to the deep-sea environment.
Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Acta Oceanologica Sinica 34 (2015):92-113, doi:10.1007/s13131-015-0650-7.
Suggested CitationPreprint: Huo, Yingyi, Cheng, Hong, Post, Anton F., Wang, Chunsheng, Jiang, Xiawei, Pan, Jie, Wu, Min, Xu, Xuewei, "Ecological functions of uncultured microorganisms in the cobalt-rich ferromanganese crust of a seamount in the central Pacific are elucidated by fosmid sequencing", 2014-07, https://doi.org/10.1007/s13131-015-0650-7, https://hdl.handle.net/1912/7304
Showing items related by title, author, creator and subject.
A 2-D tomographic model of the Juan de Fuca plate from accretion at axial seamount to subduction at the Cascadia margin from an active source ocean bottom seismometer survey Horning, Gregory W.; Canales, J. Pablo; Carbotte, Suzanne M.; Han, Shuoshuo; Carton, Helene; Nedimovic, Mladen R.; van Keken, Peter E. (John Wiley & Sons, 2016-08-14)We report results from a wide-angle controlled source seismic experiment across the Juan de Fuca plate designed to investigate the evolution of the plate from accretion at the Juan de Fuca ridge to subduction at the Cascadia ...
Arctic deep water ferromanganese-oxide deposits reflect the unique characteristics of the Arctic Ocean Hein, James R.; Konstantinova, Natalia; Mikesell, Mariah; Mizell, Kira; Fitzsimmons, Jessica N.; Lam, Phoebe J.; Jensen, Laramie T.; Xiang, Yang; Gartman, Amy; Cherkashov, Georgy; Hutchinson, Deborah R.; Till, Claire P. (John Wiley & Sons, 2017-11-08)Little is known about marine mineral deposits in the Arctic Ocean, an ocean dominated by continental shelf and basins semi-closed to deep-water circulation. Here, we present data for ferromanganese crusts and nodules ...
Vanadium isotope and elemental concentration analyses of numerous ferromanganese crusts and nodule samples Owens, Jeremy D.; Nielsen, Sune G. (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: firstname.lastname@example.org, 2020-08-13)This vanadium isotope and elemental concentration dataset analyzes numerous ferromanganese crusts and nodule samples. For a complete list of measurements, refer to the full dataset description in the supplemental file ...