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dc.contributor.authorStorace, Douglas A.  Concept link
dc.contributor.authorBraubach, Oliver R.  Concept link
dc.contributor.authorJin, Lei  Concept link
dc.contributor.authorCohen, Lawrence B.  Concept link
dc.contributor.authorSung, Uhna  Concept link
dc.identifier.citationScientific Reports 5 (2015): 10212en_US
dc.description© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 5 (2015): 10212, doi:10.1038/srep10212.en_US
dc.description.abstractUnderstanding the roles of different cell types in the behaviors generated by neural circuits requires protein indicators that report neural activity with high spatio-temporal resolution. Genetically encoded fluorescent protein (FP) voltage sensors, which optically report the electrical activity in distinct cell populations, are, in principle, ideal candidates. Here we demonstrate that the FP voltage sensor ArcLight reports odor-evoked electrical activity in the in vivo mammalian olfactory bulb in single trials using both wide-field and 2-photon imaging. ArcLight resolved fast odorant-responses in individual glomeruli, and distributed odorant responses across a population of glomeruli. Comparisons between ArcLight and the protein calcium sensors GCaMP3 and GCaMP6f revealed that ArcLight had faster temporal kinetics that more clearly distinguished activity elicited by individual odorant inspirations. In contrast, the signals from both GCaMPs were a saturating integral of activity that returned relatively slowly to the baseline. ArcLight enables optical electrophysiology of mammalian neuronal population activity in vivo.en_US
dc.description.sponsorshipSupported by US NIH DC005259, WCI 2009-003 from the National Research Foundation of Korea, a James Hudson Brown – Alexander Brown Coxe fellowship from Yale University, and a Ruth L. Kirschstein National Research Service Award DC012981.en_US
dc.publisherNature Publishing Groupen_US
dc.rightsAttribution 4.0 International*
dc.titleMonitoring brain activity with protein voltage and calcium sensorsen_US

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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International