An intensive laboratory course that provides a unique interdisciplinary training environment at the interface between cellular and computational biology.

Recent Submissions

  • Variation in traction forces during cell cycle progression 

    Vianay, Benoit; Senger, Fabrice; Alamos, Simon; Anjur-Dietrich, Maya; Bearce, Elizabeth; Cheeseman, Bevan; Lee, Lisa; Théry, Manuel (2017-10-31)
    Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of forces on cell proliferation has been fairly well characterized, the inverse relationship is much less ...
  • Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration 

    Nordenfelt, Pontus; Moore, Travis I.; Mehta, Shalin B.; Kalappurakkal, Joseph Mathew; Swaminathan, Vinay; Koga, Nobuyasu; Lambert, Talley J.; Baker, David; Waters, Jennifer C.; Oldenbourg, Rudolf; Tani, Tomomi; Mayor, Satyajit; Waterman, Clare M.; Springer, Timothy (Nature Publishing Group, 2017-12-11)
    Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts ...
  • Cell culture protocols, HeLa and CHO cells Woods Hole Physiology Course, 2006 

    Altman, David (Marine Biological Laboratory, 2006)
  • RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage–induced cell senescence 

    Cekan, Pavol; Hasegawa, Keisuke; Pan, Yu; Tubman, Emily; Odde, David; Chen, Jin-Qiu; Herrmann, Michelle A.; Kumar, Sheetal; Kalab, Petr (American Society for Cell Biology, 2016-02-10)
    The coordination of cell cycle progression with the repair of DNA damage supports the genomic integrity of dividing cells. The function of many factors involved in DNA damage response (DDR) and the cell cycle depends on ...
  • DNA damage induces nuclear actin filament assembly by Formin-2 and Spire-1/2 that promotes efficient DNA repair 

    Belin, Brittany J.; Lee, Terri; Mullins, R. Dyche (eLife Sciences Publications, 2015-08-19)
    Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates ...
  • Spindle-to-cortex communication in cleaving, polyspermic Xenopus eggs 

    Field, Christine M.; Groen, Aaron C.; Nguyen, Phuong A.; Mitchison, Timothy J. (American Society for Cell Biology, 2015-10-15)
    Mitotic spindles specify cleavage planes in early embryos by communicating their position and orientation to the cell cortex using microtubule asters that grow out from the spindle poles during anaphase. Chromatin also ...
  • The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation 

    Carvalhal, Sara; Ribeiro, Susana Abreu; Arocena, Miguel; Kasciukovic, Taciana; Temme, Achim; Koehler, Katrin; Huebner, Angela; Griffis, Eric R. (American Society for Cell Biology, 2015-08-05)
    The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which ...
  • Image analysis for biology 

    Kueh, Hao Yuan; Marco, Eugenio; Springer, Mike; Sivaramakrishnan, Sivaraj (2008-06)
  • Quantitative biology : where modern biology meets physical sciences 

    Shekhar, Shashank; Zhu, Lian; Mazutis, Linas; Sgro, Allyson E.; Fai, Thomas G.; Podolski, Marija (American Society for Cell Biology, 2014-11-05)
    Quantitative methods and approaches have been playing an increasingly important role in cell biology in recent years. They involve making accurate measurements to test a predefined hypothesis in order to compare experimental ...
  • Quantitative analysis and modeling of katanin function in flagellar length control 

    Kannegaard, Elisa; Rego, E. Hesper; Schuck, Sebastian; Feldman, Jessica L.; Marshall, Wallace F. (American Society for Cell Biology, 2014-08-20)
    Flagellar length control in Chlamydomonas reinhardtii provides a simple model system in which to investigate the general question of how cells regulate organelle size. Previous work demonstrated that Chlamydomonas cytoplasm ...
  • Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol 

    Mitchison, Timothy J.; Nguyen, Phuong A.; Coughlin, Margaret; Groen, Aaron C. (American Society for Cell Biology, 2013-03-20)
    Previous study of self-organization of Taxol-stabilized microtubules into asters in Xenopus meiotic extracts revealed motor-dependent organizational mechanisms in the spindle. We revisit this approach using clarified cytosol ...
  • Visualization of actin filaments and monomers in somatic cell nuclei 

    Belin, Brittany J.; Cimini, Beth A.; Blackburn, Elizabeth H.; Mullins, R. Dyche (American Society for Cell Biology, 2013-02-27)
    In addition to its long-studied presence in the cytoplasm, actin is also found in the nuclei of eukaryotic cells. The function and form (monomer, filament, or noncanonical oligomer) of nuclear actin are hotly debated, and ...
  • Actin binding to WH2 domains regulates nuclear import of the multifunctional actin regulator JMY 

    Zuchero, J. Bradley; Belin, Brittany J.; Mullins, R. Dyche (American Society for Cell Biology, 2012-01-19)
    Junction-mediating and regulatory protein (JMY) is a regulator of both transcription and actin filament assembly. In response to DNA damage, JMY accumulates in the nucleus and promotes p53-dependent apoptosis. JMY's ...
  • Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation 

    Pasapera, Ana M.; Schneider, Ian C.; Rericha, Erin; Schlaepfer, David D.; Waterman, Clare M. (Rockefeller University Press, 2010-03-22)
    Focal adhesions (FAs) are mechanosensitive adhesion and signaling complexes that grow and change composition in response to myosin II–mediated cytoskeletal tension in a process known as FA maturation. To understand ...
  • Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells 

    Vale, Ronald D.; Spudich, James A.; Griffis, Eric R. (Rockefeller University Press, 2009-08)
    Signals from the mitotic spindle during anaphase specify the location of the actomyosin contractile ring during cytokinesis, but the detailed mechanism remains unresolved. Here, we have imaged the dynamics of green fluorescent ...
  • Augmin : a protein complex required for centrosome-independent microtubule generation within the spindle 

    Goshima, Gohta; Mayer, Mirjam; Zhang, Nan; Stuurman, Nico; Vale, Ronald D. (Rockefeller University Press, 2008-04-28)
    Since the discovery of γ-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome. However, mislocalization of {gamma}-tubulin away from the centrosome does not inhibit mitotic spindle ...
  • Length control of the metaphase spindle 

    Goshima, Gohta; Wollman, Roy; Stuurman, Nico; Scholey, Jonathan M.; Vale, Ronald D. (2005-09-30)
    The pole-to-pole distance of the metaphase spindle is reasonably constant in a given cell type; in the case of vertebrate female oocytes, this steady-state length can be maintained for substantial lengths of time, during ...