Distal regeneration involves the age dependent activity of branchial sac stem cells in the ascidian Ciona intestinalis
Jeffery, William R.
MetadataShow full item record
Tunicates have high capacities for regeneration but the underlying mechanisms and their relationship to life cycle progression are not well understood. Here we investigate the regeneration of distal structures in the ascidian tunicate Ciona intestinalis. Analysis of regenerative potential along the proximal−distal body axis indicated that distal organs, such as the siphons, their pigmented sensory organs, and the neural complex, could only be replaced from body fragments containing the branchial sac. Distal regeneration involves the formation of a blastema composed of cells that undergo cell proliferation prior to differentiation and cells that differentiate without cell proliferation. Both cell types originate in the branchial sac and appear in the blastema at different times after distal injury. Whereas the branchial sac stem cells are present in young animals, they are depleted in old animals that have lost their regeneration capacity. Thus Ciona adults contain a population of age-related stem cells located in the branchial sac that are a source of precursors for distal body regeneration.
© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Regeneration 2 (2015): 1-18, doi:10.1002/reg2.26.
Suggested CitationArticle: Jeffery, William R., "Distal regeneration involves the age dependent activity of branchial sac stem cells in the ascidian Ciona intestinalis", Regeneration 2 (2015): 1-18, DOI:10.1002/reg2.26, https://hdl.handle.net/1912/7263
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Auger, Helene; Sasakura, Yasunori; Joly, Jean-Stephane; Jeffery, William R. (2009-12-06)Ascidians have powerful capacities for regeneration but the underlying mechanisms are poorly understood. Here we examine oral siphon regeneration in the solitary ascidian Ciona intestinalis. Following amputation, the ...
Evolution of the chordate regeneration blastema : differential gene expression and conserved role of notch signaling during siphon regeneration in the ascidian Ciona Hamada, Mayuko; Goricki, Spela; Byerly, Mardi S.; Satoh, Noriyuki; Jeffery, William R. (2015-06)The regeneration of the oral siphon (OS) and other distal structures in the ascidian Ciona intestinalis occurs by epimorphosis involving the formation of a blastema of proliferating cells. Despite the longstanding use of ...
Myelin tetraspan family proteins but no non-tetraspan family proteins are present in the ascidian (Ciona intestinalis) genome Gould, Robert M.; Morrison, Hilary G.; Gilland, Edwin; Campbell, Robert K. (Marine Biological Laboratory, 2005-08)Several of the proteins used to form and maintain myelin sheaths in the central nervous system (CNS) and the peripheral nervous system (PNS) are shared among different vertebrate classes. These proteins include one-to-several ...