Interactions between calanoid copepod hosts and their associated microbiota
Almada, Amalia A.
MetadataShow full item record
KeywordDelaware II (Ship) Cruise; Albatross IV (Ship) Cruise; Calanus finmarchicus; Phytopathogenic microorganisms
Copepods are highly abundant environmental reservoirs of many bacterial pathogens. This thesis investigates whether copepod physiology influences the abundance and community structure of its microbiome. To this end, we first examined the ability of the oceanic copepod Calanus finmarchicus to transcriptionally respond to mild stressors and demonstrated that heat shock proteins (Hsps) are a conserved element of the copepod’s regulation of stressful conditions and diapause. We then investigated the transcriptomic response of an estuarine copepod Eurytemora affinis to two distinct Vibrio species, a free-living (V. ordalii 12B09) and a zooplankton specialist (V. sp. F10 9ZB36), and found that E. affinis distinctly responds to colonizing Vibrios. Finally, we probed how specifically and predictably bacterial communities assemble on C. finmarchicus. Our findings suggest that C. finmarchicus has a predictable “core microbiome” that persists throughout the host’s entrance into diapause. Furthermore, the structure of the copepod microbiome may be driven by a combination of the copepod’s feeding history, body size, and bacterial interactions. This thesis work highlights the role of copepods as dynamic reservoirs of diverse microbial communities and implicates copepod host physiology as an important contributor to the activity, abundance, and community structure of its often pathogenic microbiota.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2015
Suggested CitationThesis: Almada, Amalia A., "Interactions between calanoid copepod hosts and their associated microbiota", 2015-02, DOI:10.1575/1912/7181, https://hdl.handle.net/1912/7181
Showing items related by title, author, creator and subject.
The ecology of colonial radiolarians : their colony morphology, trophic interactions and associations, behavior, distribution, and the photosynthesis of their symbionts Swanberg, Neil Ralph (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1979-08)Colonial radiolarians (Spumellaria) are among the most common and abundant large zooplankton, but they have been little studied by modern biologists. Colonies were found on 98% of epipelagic diving stations in the period ...
A determination of air-sea gas exchange and upper ocean biological production from five noble gases and tritiugenic helium-3 Stanley, Rachel H. R. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2007-09)The five noble gases (helium, neon, argon, krypton, and xenon) are biologically and chemically inert, making them ideal oceanographic tracers. Additionally, the noble gases have a wide range of solubilities and molecular ...
Elkins, Lynne J. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2009-02)To explore the ability of melting mafic lithologies to produce alkaline ocean-island basalts (OIB), an experimental study was carried out measuring clinopyroxene (Cpx)- melt and garnet (Gt)-melt partition coefficients ...