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dc.contributor.authorTulin, Sarah  Concept link
dc.contributor.authorBarsi, Julius C.  Concept link
dc.contributor.authorBocconcelli, Carlo  Concept link
dc.contributor.authorSmith, Joel  Concept link
dc.date.accessioned2015-01-19T22:13:35Z
dc.date.available2015-08-01T08:37:20Z
dc.date.issued2015-01-19
dc.identifier.urihttps://hdl.handle.net/1912/7101
dc.descriptionThis database contains the sequencing reads and mapped reads for GRIPseq 24hpf for S. Purpurats. README.txt is a guide to using the files in the database.en_US
dc.description.abstractThis dataset is the result of a new method for identifying putative cis-regulatory elements genome wide, termed GRIPseq for Genome-wide Regulatory element Immunoprecipitation. Our protocol combines elements of chromatin conformation capture (e.g., 3C, 4C, Hi-C), chromatin immunoprecipitation, and paired-end high-throughput sequencing with molecular tools that enrich for active cis-regulatory elements across the genome. Our pioneer dataset, available to the community, derives from the purple sea urchin, Stronglyocentrotus purpuratus, mesenchyme blastula embryos collected at 24 hpf. We benchmark our results against independent findings, i.e., rigorously tested cis-elements previously identified and find good congruence at the loci we have investigated, and in particular we find a strong signal-to-noise ratio for known distal elements, marking an improvement over existing methods. A corollary of this approach is the ability, in many cases, to link cis-regulatory elements to the genes they regulate.en_US
dc.format.mimetypeapplication/zip
dc.format.mimetypetext/plain
dc.language.isoen_USen_US
dc.relation.ispartofhttps://hdl.handle.net/1912/8259
dc.subjectgene regulationen_US
dc.subjecttranscription factor binding siteen_US
dc.subjectPol IIen_US
dc.subjectChIPseqen_US
dc.subjectsea urchinen_US
dc.titleGRIPseq data for S. Purpuratus 24 hpfen_US
dc.typeDataseten_US
dc.description.embargo2015-08-01en_US
dc.identifier.doi10.1575/1912/7101


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