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dc.contributor.authorSchmidt, Victor T.  Concept link
dc.contributor.authorReveillaud, Julie  Concept link
dc.contributor.authorZettler, Erik R.  Concept link
dc.contributor.authorMincer, Tracy J.  Concept link
dc.contributor.authorMurphy, Leslie G.  Concept link
dc.contributor.authorAmaral-Zettler, Linda A.  Concept link
dc.date.accessioned2015-01-08T15:20:24Z
dc.date.available2015-01-08T15:20:24Z
dc.date.issued2014-11-13
dc.identifier.citationFrontiers in Microbiology 5 (2014): 563en_US
dc.identifier.urihttps://hdl.handle.net/1912/7029
dc.description© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 5 (2014): 563, doi:10.3389/fmicb.2014.00563.en_US
dc.description.abstractThe genus Vibrio is a metabolically diverse group of facultative anaerobic bacteria, common in aquatic environments and marine hosts. The genus contains several species of importance to human health and aquaculture, including the causative agents of human cholera and fish vibriosis. Vibrios display a wide variety of known life histories, from opportunistic pathogens to long-standing symbionts with individual host species. Studying Vibrio ecology has been challenging as individual species often display a wide range of habitat preferences, and groups of vibrios can act as socially cohesive groups. Although strong associations with salinity, temperature and other environmental variables have been established, the degree of habitat or host specificity at both the individual and community levels is unknown. Here we use oligotyping analyses in combination with a large collection of existing Vibrio 16S ribosomal RNA (rRNA) gene sequence data to reveal patterns of Vibrio ecology across a wide range of environmental, host, and abiotic substrate associated habitats. Our data show that individual taxa often display a wide range of habitat preferences yet tend to be highly abundant in either substrate-associated or free-living environments. Our analyses show that Vibrio communities share considerable overlap between two distinct hosts (i.e., sponge and fish), yet are distinct from the abiotic plastic substrates. Lastly, evidence for habitat specificity at the community level exists in some habitats, despite considerable stochasticity in others. In addition to providing insights into Vibrio ecology across a broad range of habitats, our study shows the utility of oligotyping as a facile, high-throughput and unbiased method for large-scale analyses of publically available sequence data repositories and suggests its wide application could greatly extend the range of possibilities to explore microbial ecology.en_US
dc.description.sponsorshipThis work was supported by an NSF Collaborative grant to Erik Zettler (OCE-1155379), Tracy J. Mincer (OCE-1155671) and Linda A. Amaral-Zettler (OCE-1155571), NSF TUES grant to Erik Zettler and Linda A. Amaral-Zettler (DUE-1043468). Additional support came from the Woods Hole Center for Oceans and Human Health from the National Institutes of Health and National Science Foundation (NIH/NIEHS 1 P50 ES012742-01 and NSF/OCE 0430724-J: Linda A. Amaral-Zettler and Leslie Murphy) and an NSF/OCE-1128039 award (Linda A. Amaral-Zettler and Leslie Murphy). Victor Schmidt was supported during this work by an NSF IGERT fellowship (DGE 0966060, Dr. David Rand, PI).en_US
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/vnd.ms-excel
dc.format.mimetypeapplication/zip
dc.format.mimetypeapplication/msword
dc.language.isoen_USen_US
dc.publisherFrontiers Mediaen_US
dc.relation.urihttps://doi.org/10.3389/fmicb.2014.00563
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectOligotypingen_US
dc.subjectVibrio ecologyen_US
dc.subjectHost-microbe interactionsen_US
dc.subjectIllumina sequencingen_US
dc.subject16S rRNA analysisen_US
dc.subjectPlastisphereen_US
dc.subjectAquaculture pathogensen_US
dc.subjectMeta-analysisen_US
dc.titleOligotyping reveals community level habitat selection within the genus Vibrioen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2014.00563


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International