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dc.contributor.authorSharma, Adrian K.  Concept link
dc.contributor.authorBecker, Jamie W.  Concept link
dc.contributor.authorOttesen, Elizabeth A.  Concept link
dc.contributor.authorBryant, Jessica A.  Concept link
dc.contributor.authorDuhamel, Solange  Concept link
dc.contributor.authorKarl, David M.  Concept link
dc.contributor.authorCordero, Otto X.  Concept link
dc.contributor.authorRepeta, Daniel J.  Concept link
dc.contributor.authorDeLong, Edward F.  Concept link
dc.date.accessioned2014-10-29T16:08:18Z
dc.date.available2014-10-29T16:08:18Z
dc.date.issued2013-07
dc.identifier.urihttps://hdl.handle.net/1912/6931
dc.descriptionAuthor Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in Environmental Microbiology 16 (2014): 2815-2830, doi:10.1111/1462-2920.12254.en_US
dc.description.abstractA considerable fraction of the Earth's organic carbon exists in dissolved form in seawater. To investigate the roles of planktonic marine microbes in the biogeochemical cycling of this dissolved organic matter (DOM), we performed controlled seawater incubation experiments and followed the responses of an oligotrophic surface water microbial assemblage to perturbations with DOM derived from an axenic culture of Prochlorococcus, or high-molecular weight DOM concentrated from nearby surface waters. The rapid transcriptional responses of both Prochlorococcus and Pelagibacter populations suggested the utilization of organic nitrogen compounds common to both DOM treatments. Along with these responses, both populations demonstrated decreases in gene transcripts associated with nitrogen stress, including those involved in ammonium acquisition. In contrast, responses from low abundance organisms of the NOR5/OM60 gammaproteobacteria were observed later in the experiment, and included elevated levels of gene transcripts associated with polysaccharide uptake and oxidation. In total, these results suggest that numerically dominant oligotrophic microbes rapidly acquire nitrogen from commonly available organic sources, and also point to an important role for carbohydrates found within the DOM pool for sustaining the less abundant microorganisms in these oligotrophic systems.en_US
dc.description.sponsorshipThis work was supported by a National Science Foundation Science and Technology Center Award EF0424599 (E.F.D and D.M.K.), grants to D.M.K., D.J.R and E.F.D from the Gordon and Betty Moore Foundation, a gift from the Agouron Institute (to E.F.D.) and a fellowship (202180) to A.K.S. from the Canadian Institutes of Health Research (CIHR).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1111/1462-2920.12254
dc.titleDistinct dissolved organic matter sources induce rapid transcriptional responses in coexisting populations of Prochlorococcus, Pelagibacter and the OM60 cladeen_US
dc.typePreprinten_US


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