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dc.contributor.authorThomas, François  Concept link
dc.contributor.authorGiblin, Anne E.  Concept link
dc.contributor.authorCardon, Zoe G.  Concept link
dc.contributor.authorSievert, Stefan M.  Concept link
dc.date.accessioned2014-08-15T17:30:02Z
dc.date.available2014-08-15T17:30:02Z
dc.date.issued2014-06-23
dc.identifier.citationFrontiers in Microbiology 5 (2014): 309en_US
dc.identifier.urihttps://hdl.handle.net/1912/6807
dc.description© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 5 (2014): 309, doi:10.3389/fmicb.2014.00309.en_US
dc.description.abstractSalt marshes are highly productive ecosystems hosting an intense sulfur (S) cycle, yet little is known about S-oxidizing microorganisms in these ecosystems. Here, we studied the diversity and transcriptional activity of S-oxidizers in salt marsh sediments colonized by the plant Spartina alterniflora, and assessed variations with sediment depth and small-scale compartments within the rhizosphere. We combined next-generation amplicon sequencing of 16S rDNA and rRNA libraries with phylogenetic analyses of marker genes for two S-oxidation pathways (soxB and rdsrAB). Gene and transcript numbers of soxB and rdsrAB phylotypes were quantified simultaneously, using newly designed (RT)-qPCR assays. We identified a diverse assemblage of S-oxidizers, with Chromatiales and Thiotrichales being dominant. The detection of transcripts from S-oxidizers was mostly confined to the upper 5 cm sediments, following the expected distribution of root biomass. A common pool of species dominated by Gammaproteobacteria transcribed S-oxidation genes across roots, rhizosphere, and surrounding sediment compartments, with rdsrAB transcripts prevailing over soxB. However, the root environment fine-tuned the abundance and transcriptional activity of the S-oxidizing community. In particular, the global transcription of soxB was higher on the roots compared to mix and rhizosphere samples. Furthermore, the contribution of Epsilonproteobacteria-related S-oxidizers tended to increase on Spartina roots compared to surrounding sediments. These data shed light on the under-studied oxidative part of the sulfur cycle in salt marsh sediments and indicate small-scale heterogeneities are important factors shaping abundance and potential activity of S-oxidizers in the rhizosphere.en_US
dc.description.sponsorshipThis work was supported by NSF Ecosystems Studies grants DEB-1050557 (Stefan M. Sievert) and DEB-1050713 (Zoe G. Cardon, Anne E. Giblin). Logistical support was provided by the PIE-LTER (NSF grant OCE—1238212).en_US
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/msword
dc.language.isoen_USen_US
dc.publisherFrontiers Mediaen_US
dc.relation.urihttps://doi.org/10.3389/fmicb.2014.00309
dc.rightsAttribution 3.0 Unported
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.titleRhizosphere heterogeneity shapes abundance and activity of sulfur-oxidizing bacteria in vegetated salt marsh sedimentsen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2014.00309


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Attribution 3.0 Unported
Except where otherwise noted, this item's license is described as Attribution 3.0 Unported