The microbiome in pediatric cystic fibrosis patients : the role of shared environment suggests a window of intervention
Hampton, Thomas H.
Green, Deanna M.
Cutting, Garry R.
Morrison, Hilary G.
Sogin, Mitchell L.
Gifford, Alex H.
Stanton, Bruce A.
O’Toole, George A.
MetadataShow full item record
Cystic fibrosis (CF) is caused by mutations in the CFTR gene that predispose the airway to infection. Chronic infection by pathogens such as Pseudomonas aeruginosa leads to inflammation that gradually degrades lung function, resulting in morbidity and early mortality. In a previous study of CF monozygotic twins, we demonstrate that genetic modifiers significantly affect the establishment of persistent P. aeruginosa colonization in CF. Recognizing that bacteria other than P. aeruginosa contribute to the CF microbiome and associated pathology, we used deep sequencing of sputum from pediatric monozygotic twins and nontwin siblings with CF to characterize pediatric bacterial communities and the role that genetics plays in their evolution. We found that the microbial communities in sputum from pediatric patients living together were much more alike than those from pediatric individuals living apart, regardless of whether samples were taken from monozygous twins or from nontwin CF siblings living together, which we used as a proxy for dizygous twins. In contrast, adult communities were comparatively monolithic and much less diverse than the microbiome of pediatric patients. Taken together, these data and other recent studies suggest that as patients age, the CF microbiome becomes less diverse, more refractory to treatment and dominated by mucoid P. aeruginosa, as well as being associated with accelerated pulmonary decline. Our studies show that the microbiome of pediatric patients is susceptible to environmental influences, suggesting that interventions to preserve the community structure found in young CF patients might be possible, perhaps slowing disease progression.
© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Microbiome 2 (2014): 14, doi:10.1186/2049-2618-2-14.
Suggested CitationArticle: Hampton, Thomas H., Green, Deanna M., Cutting, Garry R., Morrison, Hilary G., Sogin, Mitchell L., Gifford, Alex H., Stanton, Bruce A., O’Toole, George A., "The microbiome in pediatric cystic fibrosis patients : the role of shared environment suggests a window of intervention", Microbiome 2 (2014): 14, DOI:10.1186/2049-2618-2-14, https://hdl.handle.net/1912/6784
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Iron supplementation does not worsen respiratory health or alter the sputum microbiome in cystic fibrosis Gifford, Alex H.; Alexandru, Diana M.; Li, Zhigang; Dorman, Dana B.; Moulton, Lisa A.; Price, Katherine E.; Hampton, Thomas H.; Sogin, Mitchell L.; Zuckerman, Jonathan B.; Parker, H. Worth; Stanton, Bruce A.; O'Toole, George A. (2013-10)Iron supplementation for hypoferremic anemia could potentiate bacterial growth in the cystic fibrosis (CF) lung, but clinical trials testing this hypothesis are lacking. Twenty-two adults with CF and hypoferremic anemia ...
Analysis of lung microbiota in bronchoalveolar lavage, protected brush and sputum samples from subjects with mild-to-moderate cystic fibrosis Lung Disease Hogan, Deborah A.; Willger, Sven D.; Dolben, Emily L.; Hampton, Thomas H.; Stanton, Bruce A.; Morrison, Hilary G.; Sogin, Mitchell L.; Czum, Julianna; Ashare, Alix (Public Library of Science, 2016-03-04)Individuals with cystic fibrosis (CF) often acquire chronic lung infections that lead to irreversible damage. We sought to examine regional variation in the microbial communities in the lungs of individuals with mild-to-moderate ...
Aquarium microbiome response to ninety-percent system water change : clues to microbiome management Van Bonn, William; LaPointe, Allen; Gibbons, Sean M.; Frazier, Angel; Hampton-Marcell, Jarrad T.; Gilbert, Jack A. (2015-04)The bacterial community composition and structure of water from an established teleost fish system was examined before, during and after a major water change to explore the impact of such a water-change disturbance on the ...