Better to light a candle than curse the darkness : illuminating spatial localization and temporal dynamics of rapid microbial growth in the rhizosphere
Herron, Patrick M.
Gage, Daniel J.
Pinedo, Catalina Arango
Haider, Zane K.
Cardon, Zoe G.
MetadataShow full item record
KeywordRhizosphere; Microbiosensor; Lux; Roots; Pseudomonas; Zeamays; Solanumlycopersicum; Populus nigra
The rhizosphere is a hotbed of microbial activity in ecosystems, fueled by carbon compounds from plant roots. Basic questions about the location and dynamics of plant-spurred microbial growth in the rhizosphere are difficult to answer with standard, destructive soil assays mixing a multitude of microbe-scale microenvironments in a single, often sieved, sample. Soil microbial biosensors designed with the luxCDABE reporter genes fused to a promoter of interest enable continuous imaging of the microbial perception of (and response to) environmental conditions in soil. We used the common soil bacterium Pseudomonas putida KT2440 as host to plasmid pZKH2 containing a fusion between the strong constitutive promoter nptII and luxCDABE (coding for light-emitting proteins) from Vibrio fischeri. Experiments in liquid media demonstrated that high light production by KT2440/pZKH2 was associated with rapid microbial growth supported by high carbon availability. We applied the biosensors in microcosms filled with non-sterile soil in which corn (Zea mays L.), black poplar (Populus nigra L.), or tomato (Solanum lycopersicum L.) was growing. We detected minimal light production from microbiosensors in the bulk soil, but biosensors reported continuously from around roots for as long as six days. For corn, peaks of luminescence were detected 1–4 and 20–35 mm along the root axis behind growing root tips, with the location of maximum light production moving farther back from the tip as root growth rate increased. For poplar, luminescence around mature roots increased and decreased on a coordinated diel rhythm, but was not bright near root tips. For tomato, luminescence was dynamic, but did not exhibit a diel rhythm, appearing in acropetal waves along roots. KT2440/pZKH2 revealed that root tips are not always the only, or even the dominant, hotspots for rhizosphere microbial growth, and carbon availability is highly variable in space and time around roots. - See more at: http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00323/full#sthash.Bv7U0hD6.dpuf
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Plant Science 4 (2013): 323, doi:10.3389/fpls.2013.00323.
Suggested CitationArticle: Herron, Patrick M., Gage, Daniel J., Pinedo, Catalina Arango, Haider, Zane K., Cardon, Zoe G., "Better to light a candle than curse the darkness : illuminating spatial localization and temporal dynamics of rapid microbial growth in the rhizosphere", Frontiers in Plant Science 4 (2013): 323, DOI:10.3389/fpls.2013.00323, https://hdl.handle.net/1912/6489
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Diel plant water use and competitive soil cation exchange interact to enhance NH4+ and K+ availability in the rhizosphere Espeleta, Javier F.; Cardon, Zoe G.; Mayer, K. Ulrich; Neumann, Rebecca B. (Springer, 2016-11-12)Hydro-biogeochemical processes in the rhizosphere regulate nutrient and water availability, and thus ecosystem productivity. We hypothesized that two such processes often neglected in rhizosphere models — diel plant water ...
Rhizosphere heterogeneity shapes abundance and activity of sulfur-oxidizing bacteria in vegetated salt marsh sediments Thomas, François; Giblin, Anne E.; Cardon, Zoe G.; Sievert, Stefan M. (Frontiers Media, 2014-06-23)Salt marshes are highly productive ecosystems hosting an intense sulfur (S) cycle, yet little is known about S-oxidizing microorganisms in these ecosystems. Here, we studied the diversity and transcriptional activity of ...
Wheat rhizosphere harbors a less complex and more stable microbial co-occurrence pattern than bulk soil Fan, Kunkun; Weisenhorn, Pamela B.; Gilbert, Jack A.; Chu, Haiyan (2018-07-25)The rhizosphere harbors complex microbial communities, whose dynamic associations are considered critical for plant growth and health but remain poorly understood. We constructed co-occurrence networks for archaeal, ...