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    Species-specific relative ahr1 binding affinities of 2,3,4,7,8-pentachlorodibenzofuran explain avian species differences in its relative potency

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    Farmahin et al-Revised-clean copy .pdf (440.6Kb)
    Date
    2013-12
    Author
    Farmahin, Reza  Concept link
    Jones, Stephanie P.  Concept link
    Crump, Doug  Concept link
    Hahn, Mark E.  Concept link
    Giesy, John P.  Concept link
    Zwiernik, Matthew J.  Concept link
    Bursian, Steven J.  Concept link
    Kennedy, Sean W.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/6408
    As published
    https://doi.org/10.1016/j.cbpc.2013.12.005
    Keyword
     Aryl hydrocarbon receptor; Cell-based binding assay; Dioxin; COS-7 cells; Bird; PeCDF; TCDD 
    Abstract
    Results of recent studies showed that 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are equipotent in domestic chicken (Gallus gallus domesticus) while PeCDF is more potent than TCDD in ring-necked pheasant (Phasianus colchicus) and Japanese quail (Coturnix japonica). To elucidate the mechanism(s) underlying these differences in relative potency of PeCDF among avian species, we tested the hypothesis that this is due to species-specific differential binding affinity of PeCDF to the aryl hydrocarbon receptor 1 (AHR1). Here, we modified a cell-based binding assay that allowed us to measure the binding affinity of dioxin-like compounds (DLCs) to avian AHR1 expressed in COS-7 (fibroblast-like cells). The results of the binding assay show that PeCDF and TCDD bind with equal affinity to chicken AHR1, but PeCDF binds with greater affinity than TCDD to pheasant (3-fold) and Japanese quail (5-fold) AHR1. The current report introduces a COS-7 whole-cell binding assay and provides a mechanistic explanation for differential relative potencies of PeCDF among species of birds.
    Description
    Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 161 (2014): 21-25, doi:10.1016/j.cbpc.2013.12.005.
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    • Biology
    Suggested Citation
    Preprint: Farmahin, Reza, Jones, Stephanie P., Crump, Doug, Hahn, Mark E., Giesy, John P., Zwiernik, Matthew J., Bursian, Steven J., Kennedy, Sean W., "Species-specific relative ahr1 binding affinities of 2,3,4,7,8-pentachlorodibenzofuran explain avian species differences in its relative potency", 2013-12, https://doi.org/10.1016/j.cbpc.2013.12.005, https://hdl.handle.net/1912/6408
     

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