Species-specific relative ahr1 binding affinities of 2,3,4,7,8-pentachlorodibenzofuran explain avian species differences in its relative potency
Jones, Stephanie P.
Hahn, Mark E.
Giesy, John P.
Zwiernik, Matthew J.
Bursian, Steven J.
Kennedy, Sean W.
MetadataShow full item record
Results of recent studies showed that 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are equipotent in domestic chicken (Gallus gallus domesticus) while PeCDF is more potent than TCDD in ring-necked pheasant (Phasianus colchicus) and Japanese quail (Coturnix japonica). To elucidate the mechanism(s) underlying these differences in relative potency of PeCDF among avian species, we tested the hypothesis that this is due to species-specific differential binding affinity of PeCDF to the aryl hydrocarbon receptor 1 (AHR1). Here, we modified a cell-based binding assay that allowed us to measure the binding affinity of dioxin-like compounds (DLCs) to avian AHR1 expressed in COS-7 (fibroblast-like cells). The results of the binding assay show that PeCDF and TCDD bind with equal affinity to chicken AHR1, but PeCDF binds with greater affinity than TCDD to pheasant (3-fold) and Japanese quail (5-fold) AHR1. The current report introduces a COS-7 whole-cell binding assay and provides a mechanistic explanation for differential relative potencies of PeCDF among species of birds.
Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 161 (2014): 21-25, doi:10.1016/j.cbpc.2013.12.005.
Suggested CitationPreprint: Farmahin, Reza, Jones, Stephanie P., Crump, Doug, Hahn, Mark E., Giesy, John P., Zwiernik, Matthew J., Bursian, Steven J., Kennedy, Sean W., "Species-specific relative ahr1 binding affinities of 2,3,4,7,8-pentachlorodibenzofuran explain avian species differences in its relative potency", 2013-12, https://doi.org/10.1016/j.cbpc.2013.12.005, https://hdl.handle.net/1912/6408
Showing items related by title, author, creator and subject.
Becker, Jamie W.; Berube, Paul M.; Follett, Christopher L.; Waterbury, John B.; Chisholm, Sallie W.; DeLong, Edward F.; Repeta, Daniel J. (Frontiers Media, 2014-03-28)Production of dissolved organic matter (DOM) by marine phytoplankton supplies the majority of organic substrate consumed by heterotrophic bacterioplankton in the sea. This production and subsequent consumption converts a ...
Schistosoma mansoni : use of a fluorescent indicator to detect nitric oxide and related species in living parasites Kohn, Andrea B.; Lea, Jeanne M.; Moroz, Leonid L.; Greenberg, Robert M. (2005-12-20)Nitric oxide (NO) is synthesized enzymatically by nitric oxide synthase (NOS). Several groups have previously presented evidence for NOS activity and immunoreactivity in several parasitic platyhelminths, including ...
Species-specific abundance of bivalve larvae in relation to biological and physical conditions in a Cape Cod estuary : Waquoit Bay, Massachusetts (USA) Thompson, Christine M.; York, Richard H.; Gallager, Scott M. (2012-06)Physical and biological conditions impact recruitment and adult population structure of 34 marine invertebrates by affecting early life history processes from spawning to post-settlement. We investigated how temperature, ...