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    Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation

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    josaa-30-4-769.pdf (3.833Mb)
    Date
    2013-03-27
    Author
    Shribak, Michael  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/6398
    As published
    https://doi.org/10.1364/JOSAA.30.000769
    DOI
    10.1364/JOSAA.30.000769
    Abstract
    We describe a quantitative orientation-independent differential interference contrast (DIC) microscope, which allows bias retardation to be modulated and shear directions to be switched rapidly without any mechanical movement. The shear direction is switched by a regular liquid-crystal cell sandwiched between two standard DIC prisms. Another liquid-crystal cell modulates the bias. Techniques for measuring parameters of DIC prisms and calibrating the bias are shown. Two sets of raw DIC images with the orthogonal shear directions are captured within 1 s. Then the quantitative image of optical path gradient distribution within a thin optical section is computed. The gradient data are used to obtain a quantitative distribution of the optical path, which represents the refractive index gradient or height distribution. Computing enhanced regular DIC images with any desired shear direction is also possible.
    Description
    Author Posting. © Optical Society of America, 2013. This article is posted here by permission of Optical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Optical Society of America A: Optics, Image Science, and Vision 30 (2013): 769-782, doi:10.1364/JOSAA.30.000769.
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    • Cellular Dynamics Program
    Suggested Citation
    Journal of the Optical Society of America A: Optics, Image Science, and Vision 30 (2013): 769-782
     
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