Capture of lipopolysaccharide (endotoxin) by the blood clot : a comparative study
Armstrong, Margaret T.
Rickles, Frederick R.
Armstrong, Peter B.
MetadataShow full item record
In vertebrates and arthropods, blood clotting involves the establishment of a plug of aggregated thrombocytes (the cellular clot) and an extracellular fibrillar clot formed by the polymerization of the structural protein of the clot, which is fibrin in mammals, plasma lipoprotein in crustaceans, and coagulin in the horseshoe crab, Limulus polyphemus. Both elements of the clot function to staunch bleeding. Additionally, the extracellular clot functions as an agent of the innate immune system by providing a passive anti-microbial barrier and microbial entrapment device, which functions directly at the site of wounds to the integument. Here we show that, in addition to these passive functions in immunity, the plasma lipoprotein clot of lobster, the coagulin clot of Limulus, and both the platelet thrombus and the fibrin clot of mammals (human, mouse) operate to capture lipopolysaccharide (LPS, endotoxin). The lipid A core of LPS is the principal agent of gram-negative septicemia, which is responsible for more than 100,000 human deaths annually in the United States and is similarly toxic to arthropods. Quantification using the Limulus Amebocyte Lysate (LAL) test shows that clots capture significant quantities of LPS and fluorescent-labeled LPS can be seen by microscopy to decorate the clot fibrils. Thrombi generated in the living mouse accumulate LPS in vivo. It is suggested that capture of LPS released from gram-negative bacteria entrapped by the blood clot operates to protect against the disease that might be caused by its systemic dispersal.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 8 (2013): e80192, doi:10.1371/journal.pone.0080192.
Suggested CitationPLoS One 8 (2013): e80192
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Characterizing the deep insular shelf coral reef habitat of the Hind Bank marine conservation district (US Virgin Islands) using the Seabed autonomous underwater vehicle Armstrong, Roy A.; Singh, Hanumant; Torres, Juan; Nemeth, Richard S.; Can, Ali; Roman, Christopher N.; Eustice, Ryan M.; Riggs, Lauren; Garcia-Moliner, Graciela (2005-10-26)The benthic communities of the deep insular shelf at the Hind Bank Marine Conservation District (MCD), an important spawning grouper aggregation site, were studied with the Seabed autonomous underwater vehicle (AUV) at ...
Assessing the uncertainties of model estimates of primary productivity in the tropical Pacific Ocean Friedrichs, Marjorie A. M.; Carr, Mary-Elena; Barber, Richard T.; Scardi, Michele; Antoine, David; Armstrong, Robert A.; Asanuma, Ichio; Behrenfeld, Michael J.; Buitenhuis, Erik T.; Chai, Fei; Christian, James R.; Ciotti, Aurea M.; Doney, Scott C.; Dowell, Mark; Dunne, John P.; Gentili, Bernard; Gregg, Watson; Hoepffner, Nicolas; Ishizaka, Joji; Kameda, Takahiko; Lima, Ivan D.; Marra, John F.; Melin, Frederic; Moore, J. Keith; Morel, Andre; O'Malley, Robert T.; O'Reilly, Jay; Saba, Vincent S.; Schmeltz, Marjorie; Smyth, Tim J.; Tjiputra, Jerry; Waters, Kirk; Westberry, Toby K.; Winguth, Arne (2008-03)Depth-integrated primary productivity (PP) estimates obtained from satellite ocean color based models (SatPPMs) and those generated from biogeochemical ocean general circulation models (BOGCMs) represent a key resource ...
Storm-induced inner-continental shelf circulation and sediment transport : Long Bay, South Carolina Warner, John C.; Armstrong, Brandy; Sylvester, Charlene S.; Voulgaris, George; Nelson, Timothy R.; Schwab, William C.; Denny, Jane F. (Elsevier B.V., 2012-05-10)Long Bay is a sediment-starved, arcuate embayment located along the US East Coast connecting both South and North Carolina. In this region the rates and pathways of sediment transport are important because they determine ...