Polarized light microscopy in reproductive and developmental biology
Mehta, Shalin B.
MetadataShow full item record
The polarized light microscope reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms. Therefore, it is a powerful tool to monitor and analyze the early developmental stages of organisms that lend themselves to microscopic observations. In this article we briefly discuss the components specific to a traditional polarizing microscope and some historically important observations on chromosome packing in sperm head, first zygote division of the sea urchin, and differentiation initiated by the first uneven cell division in the sand dollar. We then introduce the LC-PolScope and describe its use for measuring birefringence and polarized fluorescence in living cells and tissues. Applications range from the enucleation of mouse oocytes to analyzing the polarized fluorescence of the water strider acrosome. We end by reporting first results on the birefringence of the developing chick brain, which we analyzed between developmental stages of days 12 through 20.
Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in Molecular Reproduction and Development (2013), doi:10.1002/mrd.22221.
Suggested CitationPreprint: Koike-Tani, Maki, Tani, Tomomi, Mehta, Shalin B., Verma, Amitabh, Oldenbourg, Rudolf, "Polarized light microscopy in reproductive and developmental biology", 2013-05, https://doi.org/10.1002/mrd.22221, https://hdl.handle.net/1912/6267
Showing items related by title, author, creator and subject.
Time lapse movie of meiosis I in a living spermatocyte from the crane fly, Nephrotoma suturalis, viewed with polarized light microscopy LaFountain, James R.; Oldenbourg, Rudolf (2002-11)The events of meiosis I in a living spermatocyte obtained from the testis of a crane-fly larva are recorded in this time-lapse sequence beginning at diakinesis through telophase to the near completion of cytokinesis following ...
Mapping birefringence in three dimensions using polarized light field microscopy : the case of the juvenile clamshell Tran, Mai Thi; Oldenbourg, Rudolf (2018-05)We report methods to generate three dimensional maps of birefringence, its position and orientation in juvenile shells of the Atlantic hard clamshell (Mercenaria mercenaria). For measuring the retardance and optic axis ...
Time lapse movie of meiosis II in a living spermatocyte from the crane fly, Nephrotoma suturalis, viewed with polarized light microscopy LaFountain, James R.; Oldenbourg, Rudolf (2002-11)The events of meiosis II in two living spermatocytes obtained from the testis of a crane-fly larva are recorded in this time-lapse sequence beginning at prophase II through telophase II to the near completion of cytokinesis ...