Polarized light microscopy in reproductive and developmental biology
Date
2013-05Author
Koike-Tani, Maki
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Tani, Tomomi
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Mehta, Shalin B.
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Verma, Amitabh
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Oldenbourg, Rudolf
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https://hdl.handle.net/1912/6267As published
https://doi.org/10.1002/mrd.22221Abstract
The polarized light microscope reveals orientational order in native molecular structures inside
living cells, tissues, and whole organisms. Therefore, it is a powerful tool to monitor and analyze
the early developmental stages of organisms that lend themselves to microscopic observations. In
this article we briefly discuss the components specific to a traditional polarizing microscope and
some historically important observations on chromosome packing in sperm head, first zygote
division of the sea urchin, and differentiation initiated by the first uneven cell division in the
sand dollar. We then introduce the LC-PolScope and describe its use for measuring birefringence
and polarized fluorescence in living cells and tissues. Applications range from the enucleation of
mouse oocytes to analyzing the polarized fluorescence of the water strider acrosome. We end by
reporting first results on the birefringence of the developing chick brain, which we analyzed
between developmental stages of days 12 through 20.
Description
Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in Molecular Reproduction and Development (2013), doi:10.1002/mrd.22221.
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Preprint: Koike-Tani, Maki, Tani, Tomomi, Mehta, Shalin B., Verma, Amitabh, Oldenbourg, Rudolf, "Polarized light microscopy in reproductive and developmental biology", 2013-05, https://doi.org/10.1002/mrd.22221, https://hdl.handle.net/1912/6267Related items
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