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dc.contributor.authorMehta, Shalin B.  Concept link
dc.contributor.authorShribak, Michael  Concept link
dc.contributor.authorOldenbourg, Rudolf  Concept link
dc.date.accessioned2013-10-17T15:53:35Z
dc.date.available2014-10-22T08:57:21Z
dc.date.issued2013-07
dc.identifier.urihttps://hdl.handle.net/1912/6266
dc.descriptionAuthor Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of IOP Publishing for personal use, not for redistribution. The definitive version was published in Journal of Optics 15 (2013): 094007, doi:10.1088/2040-8978/15/9/094007.en_US
dc.description.abstractPolarized light microscopy provides unique opportunities for analyzing the molecular order in manmade and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope was introduced as a modern version of the traditional polarizing microscope enhanced by liquid crystal devices for the control of polarization, and by electronic imaging and digital image processing for fast and comprehensive image acquisition and analysis. The LCPolScope is commonly used for birefringence imaging, analyzing the spatial and temporal variations of the differential phase delay in ordered and transparent materials. Here we describe an alternative use of the LC-PolScope for imaging the polarization dependent transmittance of dichroic materials. We explain the minor changes needed to convert the instrument between the two imaging modes, discuss the relationship between the quantities measured with either instrument, and touch on the physical connection between refractive index, birefringence, transmittance, diattenuation, and dichroism.en_US
dc.description.sponsorshipThis work was supported by a fellowship of the Human Frontiers Science Program awarded to SM and a grant from the National Institute of Biomedical Imaging and Bioengineering (grant Nr. R01EB002045) awarded to RO. MS acknowledges support from the National Institute of General Medical Sciences/NIH (grant R01-GM101701).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1088/2040-8978/15/9/094007
dc.subjectBirefringenceen_US
dc.subjectDiattenuationen_US
dc.subjectDichroismen_US
dc.subjectRetardanceen_US
dc.subjectTransmittanceen_US
dc.subjectLC-PolScopeen_US
dc.subjectPolarized lighten_US
dc.subjectLiquid-crystal compensatoren_US
dc.titlePolarized light imaging of birefringence and diattenuation at high resolution and high sensitivityen_US
dc.typePreprinten_US
dc.description.embargo2014-09-10en_US


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