Polarized light imaging of birefringence and diattenuation at high resolution and high sensitivity
MetadataShow full item record
KeywordBirefringence; Diattenuation; Dichroism; Retardance; Transmittance; LC-PolScope; Polarized light; Liquid-crystal compensator
Polarized light microscopy provides unique opportunities for analyzing the molecular order in manmade and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope was introduced as a modern version of the traditional polarizing microscope enhanced by liquid crystal devices for the control of polarization, and by electronic imaging and digital image processing for fast and comprehensive image acquisition and analysis. The LCPolScope is commonly used for birefringence imaging, analyzing the spatial and temporal variations of the differential phase delay in ordered and transparent materials. Here we describe an alternative use of the LC-PolScope for imaging the polarization dependent transmittance of dichroic materials. We explain the minor changes needed to convert the instrument between the two imaging modes, discuss the relationship between the quantities measured with either instrument, and touch on the physical connection between refractive index, birefringence, transmittance, diattenuation, and dichroism.
Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of IOP Publishing for personal use, not for redistribution. The definitive version was published in Journal of Optics 15 (2013): 094007, doi:10.1088/2040-8978/15/9/094007.
Suggested CitationPreprint: Mehta, Shalin B., Shribak, Michael, Oldenbourg, Rudolf, "Polarized light imaging of birefringence and diattenuation at high resolution and high sensitivity", 2013-07, https://doi.org/10.1088/2040-8978/15/9/094007, https://hdl.handle.net/1912/6266
Showing items related by title, author, creator and subject.
Living cells and dynamic molecules observed with the polarized light microscope : the legacy of Shinya Inoué Tani, Tomomi; Shribak, Michael; Oldenbourg, Rudolf (Marine Biological Laboratory, 2016-08)In 1948, Shinya Inoué arrived in the United States for graduate studies at Princeton. A year later he came to Woods Hole, starting a long tradition of summer research at the Marine Biological Laboratory (MBL), which quickly ...
Time lapse movie of meiosis I in a living spermatocyte from the crane fly, Nephrotoma suturalis, viewed with polarized light microscopy LaFountain, James R.; Oldenbourg, Rudolf (2002-11)The events of meiosis I in a living spermatocyte obtained from the testis of a crane-fly larva are recorded in this time-lapse sequence beginning at diakinesis through telophase to the near completion of cytokinesis following ...
Time lapse movie of meiosis II in a living spermatocyte from the crane fly, Nephrotoma suturalis, viewed with polarized light microscopy LaFountain, James R.; Oldenbourg, Rudolf (2002-11)The events of meiosis II in two living spermatocytes obtained from the testis of a crane-fly larva are recorded in this time-lapse sequence beginning at prophase II through telophase II to the near completion of cytokinesis ...