Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri
Giles, Emily C.
Berumen, Michael L.
MetadataShow full item record
Despite the ubiquitous role sponges play in reef ecosystem dynamics, little is known about population-level connectivity in these organisms. The general field of population genetics in sponges remains in its infancy. To date, microsatellite markers have only been developed for few sponge species and no sponge population genetics studies using microsatellites have been conducted in the Red Sea. Here, with the use of next-generation sequencing, we characterize 12 novel polymorphic loci for the common reef sponge, Stylissa carteri. The number of alleles per loci ranged between three and eight. Observed heterozygosity frequencies (Ho) ranged from 0.125 to 0.870, whereas expected (He) heterozygosity frequencies ranged from 0.119 to 0.812. Only one locus showed consistent deviations from Hardy-Weinberg equilibrium (HWE) in both populations and two loci consistently showed the possible presence of null alleles. No significant linkage disequilibrium was detected for any pairs of loci. These microsatellites will be of use for numerous ecological studies focused on this common and abundant sponge.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Marine Biodiversity 43 (2013): 237-241, doi:10.1007/s12526-013-0151-x.
Suggested CitationMarine Biodiversity 43 (2013): 237-241
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Finelli, Christopher; Pawlik, Joseph; McMurray, Steven (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: firstname.lastname@example.org, 2017-03-27)Benthic suspension feeders are an important component of aquatic ecosystems, as they mediate benthic-pelagic coupling and the flow of energy and nutrients. There is increasing evidence that sponges are particularly important ...
Sponge density and morphology and percent cover of sponges and associated community at Virgin Islands Territorial Coral Reef Monitoring Program site, pre- and post-hurricane, 2015-2017 Gochfeld, Deborah J; Brandt, Marilyn; Olson, Julie (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: email@example.com, 2020-07-08)Sponge density and morphology and percent cover of sponges and associated benthic community determined from videos of three transects at Virgin Islands Territorial Coral Reef Monitoring Program site, pre- and post-hurricane, ...
Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.) Banguera-Hinestroza, Eulalia; Saenz-Agudelo, Pablo; Bayer, Till; Berumen, Michael L.; Voolstra, Christian R. (Springer, 2013-01-09)A total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified ...