Superresolution by image scanning microscopy using pixel reassignment
Sheppard, Colin J. R.
Mehta, Shalin B.
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The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.
Author Posting. © Optical Society of America, 2013. This article is posted here by permission of Optical Society of America for personal use, not for redistribution. The definitive version was published in Optics Letters 38 (2013): 2889-2892, doi:10.1364/OL.38.002889.