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dc.contributor.authorHanson, Sara J.  Concept link
dc.contributor.authorStelzer, Claus-Peter  Concept link
dc.contributor.authorMark Welch, David B.  Concept link
dc.contributor.authorLogsdon, John M.  Concept link
dc.date.accessioned2013-08-06T18:47:24Z
dc.date.available2013-08-06T18:47:24Z
dc.date.issued2013-06-19
dc.identifier.citationBMC Genomics 14 (2013): 412en_US
dc.identifier.urihttps://hdl.handle.net/1912/6148
dc.description© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in BMC Genomics 14 (2013): 412, doi:10.1186/1471-2164-14-412.en_US
dc.description.abstractSexual reproduction is a widely studied biological process because it is critically important to the genetics, evolution, and ecology of eukaryotes. Despite decades of study on this topic, no comprehensive explanation has been accepted that explains the evolutionary forces underlying its prevalence and persistence in nature. Monogonont rotifers offer a useful system for experimental studies relating to the evolution of sexual reproduction due to their rapid reproductive rate and close relationship to the putatively ancient asexual bdelloid rotifers. However, little is known about the molecular underpinnings of sex in any rotifer species. We generated mRNA-seq libraries for obligate parthenogenetic (OP) and cyclical parthenogenetic (CP) strains of the monogonont rotifer, Brachionus calyciflorus, to identify genes specific to both modes of reproduction. Our differential expression analysis identified receptors with putative roles in signaling pathways responsible for the transition from asexual to sexual reproduction. Differential expression of a specific copy of the duplicated cell cycle regulatory gene CDC20 and specific copies of histone H2A suggest that such duplications may underlie the phenotypic plasticity required for reproductive mode switch in monogononts. We further identified differential expression of genes involved in the formation of resting eggs, a process linked exclusively to sex in this species. Finally, we identified transcripts from the bdelloid rotifer Adineta ricciae that have significant sequence similarity to genes with higher expression in CP strains of B. calyciflorus. Our analysis of global gene expression differences between facultatively sexual and exclusively asexual populations of B. calyciflorus provides insights into the molecular nature of sexual reproduction in rotifers. Furthermore, our results offer insight into the evolution of obligate asexuality in bdelloid rotifers and provide indicators important for the use of monogononts as a model system for investigating the evolution of sexual reproduction.en_US
dc.description.sponsorshipThis work was funded by National Institutes of Health Institute of General Medical Sciences (grant number 5R01GM079484, to JML and DMW).en_US
dc.format.mimetypeapplication/fasta
dc.format.mimetypeapplication/vnd.ms-excel
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.urihttps://doi.org/10.1186/1471-2164-14-412
dc.rightsAttribution 2.0 Generic*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0*
dc.subjectEvolution of sexual reproductionen_US
dc.subjectDifferential expression analysisen_US
dc.subjectGene ontology analysisen_US
dc.subjectMeiosisen_US
dc.subjectGametogenesisen_US
dc.subjectResting eggsen_US
dc.subjectMixis inductionen_US
dc.titleComparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg productionen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1471-2164-14-412


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