Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus
Date
2005-12-20Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/603As published
https://doi.org/10.1016/j.cbpc.2005.10.013Keyword
Aryl hydrocarbon receptor (AHR); AHR repressor (AHRR); Basic helix-loop-helix Per-ARNT-Sim (bHLH-PAS); Fish; Halogenated aromatic hydrocarbons (HAH);; Polynuclear aromatic hydrocarbons (PAH); Recombinant protein; TeleostAbstract
The aryl hydrocarbon receptor (AHR) and AHR repressor (AHRR) proteins regulate gene expression in response to some halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons. The Atlantic killifish is a valuable model of the AHR signaling pathway, but antibodies are not available to fully characterize AHR and AHRR proteins. Using bacterially expressed AHRs, we developed specific and sensitive polyclonal antisera against the killifish AHR1, AHR2, and AHRR. In immunoblots, these antibodies recognized full-length killifish AHR and AHRR proteins synthesized in rabbit reticulocyte lysate, proteins expressed in mammalian cells transfected with killifish AHR and AHRR constructs, and AHR proteins in cytosol preparations from killifish tissues. Killifish AHR1 and AHR2 proteins were detected in brain, gill, kidney, heart, liver, and spleen. Antisera specifically precipitated their respective target proteins in immunoprecipitation experiments with in vitro-expressed proteins. Killifish ARNT2 co-precipitated with AHR1 and AHR2. These sensitive, specific, and versatile antibodies will be valuable to researchers investigating AHR signaling and other physiological processes involving AHR and AHRR proteins.
Description
Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 142 (2006): 85-94, doi:10.1016/j.cbpc.2005.10.013.
Collections
Suggested Citation
Preprint: Merson, Rebeka R., Franks, Diana G., Karchner, Sibel I., Hahn, Mark E., "Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus", 2005-12-20, https://doi.org/10.1016/j.cbpc.2005.10.013, https://hdl.handle.net/1912/603Related items
Showing items related by title, author, creator and subject.
-
Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line
(Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1999-12)Aryl hydrocarbon receptor (AHR) expression and activity was characterized in the teleost hepatoma cell line, PLHC-1. This work was carried out in order to gain insights into mechanisms of halogenated aromatic hydrocarbon ... -
NR3E receptors in cnidarians : a new family of steroid receptor relatives extends the possible mechanisms for ligand binding
(2018-06)Steroid hormone receptors are important regulators of development and physiology in bilaterian animals, but the role of steroid signaling in cnidarians has been contentious. Cnidarians produce steroids, including A-ring ... -
Genetic variation at aryl hydrocarbon receptor (AHR) loci in populations of Atlantic killifish (Fundulus heteroclitus) inhabiting polluted and reference habitats
(BioMed Central, 2014-01-14)The non-migratory killifish Fundulus heteroclitus inhabits clean and polluted environments interspersed throughout its range along the Atlantic coast of North America. Several populations of this species have successfully ...
